噬血细胞综合征患者的病毒标志物：在一家三级医疗机构的前瞻性研究。

Viral markers in patients with hemophagocytosis: a prospective study in a tertiary care hospital.

作者信息

Mishra Baijayantimala, Varma Neelam, Appannanavar Suma, Malhotra Pankaj, Sharma Mrinalini, Bhatnagar Anil, Ratho Radha Kanta, Varma Subhash

机构信息

Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh, Punjab, India.

出版信息

Indian J Pathol Microbiol. 2012 Apr-Jun;55(2):215-7. doi: 10.4103/0377-4929.97876.

DOI:10.4103/0377-4929.97876

PMID:22771647

Abstract

BACKGROUND

Hemophagocytic syndrome (HPS) is a rare clinicopathological condition characterized by the activation of macrophages with prominent hemophagocytosis in bone marrow and other reticulo-endothelial systems. HPS can be familial or secondary to infections including viruses.

AIM

To study the viral markers in patients with HPS.

MATERIALS AND METHODS

Serum samples of patients with HPS and control group were screened for anti EBV VCA IgM, and IgG, anti-Parvo B19 IgM, and anti-CMV IgM antibodies using commercially available ELISA kits and CMV and ParvoB19 DNA by polymerase chain reaction (PCR).

RESULTS AND DISCUSSION

The present prospective study reports the profile of viral markers in HPS cases from north India. Among the 14 HPS cases 43% (6/14) were positive for at least one viral marker tested, of which EBV was found to be the most prevalent (3/6: 50%) followed by parvovirus B19(2/6: 33%) and cytomegalovirus (1/6: 17%). Mortality was noted in 33% of virus associated HPS patients. Our study highlights the higher association of Epstein-Barr virus (EBV) with HPS as compared to other viruses along with higher rate of mortality in both parvovirus B 19 and EBV associated HPS.

摘要

背景

噬血细胞综合征（HPS）是一种罕见的临床病理状况，其特征为巨噬细胞活化，在骨髓和其他网状内皮系统中出现显著的噬血细胞现象。HPS可呈家族性或继发于包括病毒在内的感染。

目的

研究HPS患者的病毒标志物。

材料与方法

使用市售酶联免疫吸附测定（ELISA）试剂盒对HPS患者和对照组的血清样本进行抗EB病毒衣壳抗原（EBV VCA）IgM、IgG、抗细小病毒B19 IgM以及抗巨细胞病毒（CMV）IgM抗体筛查，并通过聚合酶链反应（PCR）检测CMV和细小病毒B19 DNA。

结果与讨论

本前瞻性研究报告了印度北部HPS病例的病毒标志物情况。在14例HPS病例中，43%（6/14）至少一种检测的病毒标志物呈阳性，其中EBV最为常见（3/6：50%），其次是细小病毒B19（2/6：33%）和巨细胞病毒（1/6：17%）。在33%的病毒相关性HPS患者中观察到死亡情况。我们的研究强调，与其他病毒相比，爱泼斯坦-巴尔病毒（EBV）与HPS的关联更高，同时细小病毒B19和EBV相关性HPS的死亡率也更高。