Cardiovascular Research Center (CSIC-ICCC), Hospital de la Santa Creu i Sant Pau, Av. S. Antoni M. Claret 167, 08025 Barcelona, Spain.
FASEB J. 2012 Oct;26(10):4327-36. doi: 10.1096/fj.12-207217. Epub 2012 Jul 6.
It has been demonstrated that the adipose tissue, a highly functional metabolic tissue, is a reservoir of mesenchymal stem cells. The potential use of adipose-derived stem cells (ADSCs) from white adipose tissue (WAT) for organ repair and regeneration has been considered because of their obvious benefits in terms of accessibility and quantity of available sample. However, the functional capability of ADSCs from subjects with different adiposity has not been investigated. It has been our hypothesis that ADSCs from adipose tissue of patients with metabolic syndrome and high adiposity may be functionally impaired. We report that subcutaneous WAT stromal vascular fraction (SVF) from nonobese individuals had a significantly higher number of CD90+ cells than SVF from obese patients. The isolated ADSCs from WAT of obese patients had reduced differentiation potential and were less proangiogenic. Therefore, ADSCs in adipose tissue of obese patients have lower capacity for spontaneous or therapeutic repair than ADSCs from nonobese metabolically normal individuals.
已经证实，脂肪组织是一种高度功能性的代谢组织，是间充质干细胞的储存库。由于其在获取和样本数量方面的明显优势，人们已经考虑将来源于白色脂肪组织（WAT）的脂肪来源干细胞（ADSCs）用于器官修复和再生。然而，对于不同肥胖程度的个体来源的 ADSC 的功能能力还没有进行研究。我们假设来自代谢综合征和肥胖患者的脂肪组织的 ADSC 可能在功能上受到损害。我们报告说，非肥胖个体的皮下 WAT 基质血管部分（SVF）中的 CD90+细胞数量明显高于肥胖患者的 SVF。从肥胖患者的 WAT 中分离出的 ADSC 具有降低的分化潜能，并且成血管能力较低。因此，与非肥胖代谢正常个体的 ADSC 相比，肥胖患者脂肪组织中的 ADSC 进行自发或治疗性修复的能力较低。
