Department of Neurosurgery, Institute of Functional Brain Disorders of PLA, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
J Membr Biol. 2012 Nov;245(11):675-81. doi: 10.1007/s00232-012-9436-4. Epub 2012 Jul 8.
The chronic mild stress (CMS) protocol is widely used to evoke depression-like behaviors in the laboratory. Some animals exposed to CMS are resistant to the development of anhedonia, whereas the remaining are responsive, CMS-resilient and CMS-sensitive, respectively. The aim of this study was to examine the effects of chronic stress on oxidative parameters in the rat brain. The consumption of sweet food, protein and lipid oxidation levels and superoxide dismutase and catalase activities in the rat hippocampus, cortex and cerebellum were assessed. We found a significant increase in protein peroxidation (hippocampus and cortex), a significant increase in catalase activity (cortex, hippocampus and cerebellum) and a decrease in superoxide dismutase activity (cortex, hippocampus and cerebellum) in the CMS-sensitive group compared to the CMS-resilient group and normal controls as well as an increase in lipid peroxidation (cerebellum) in the CMS-sensitive and CMS-resilient groups compared to normal controls. However, there was no significant difference in protein peroxidation (cerebellum) and lipid peroxidation (cortex and hippocampus) among the three groups. In conclusion, our results indicate that the segregation into CMS-sensitive and -resilient groups based on sucrose intake is paralleled by significant differences in oxidative parameters. CMS induces oxidative damage and alterations in the activity of antioxidants which may lead to increased oxidative damage, irrespective of the anhedonia-like status of the stressed animals.
慢性轻度应激（CMS）方案被广泛用于在实验室中诱发类似抑郁的行为。一些暴露于 CMS 的动物对快感缺失的发展具有抗性，而其余的则分别具有抗性、CMS 抗性和 CMS 敏感性。本研究旨在研究慢性应激对大鼠大脑氧化参数的影响。评估了大鼠海马体、大脑皮层和小脑中甜食消费、蛋白质和脂质氧化水平以及超氧化物歧化酶和过氧化氢酶活性。我们发现 CMS 敏感组与 CMS 抗性组和正常对照组相比，蛋白质过氧化（海马体和大脑皮层）显著增加，过氧化氢酶活性（大脑皮层、海马体和小脑）显著增加，超氧化物歧化酶活性（大脑皮层、海马体和小脑）降低，与正常对照组相比，CMS 敏感组和 CMS 抗性组的脂质过氧化（小脑）增加。然而，三组之间的小脑蛋白质过氧化（小脑）和皮质和海马体的脂质过氧化（皮质和海马体）没有显著差异。总之，我们的结果表明，根据蔗糖摄入将 CMS 分为敏感组和抗性组与氧化参数的显著差异相平行。CMS 诱导氧化损伤和抗氧化剂活性的改变，这可能导致氧化损伤增加，而不论应激动物的快感缺失状态如何。
