Vrzhesinskaia O A, Kodentsova V M, Beketova N A, Pereverzeva O G, Kosheleva O V
Vopr Pitan. 2012;81(2):51-6.
A model of the alimentary polyhypovitaminosis varying degrees basing on partitive simultaneous reduction of all vitamins in rats diet has been proposed. The model has a principal difference from other experimental models, based on complete exclusion of 1 or 2 vitamins from animal diet. The proposed model allows you to get as close to the actually observed combined deficiency of several vitamins among the population. 5-fold decrease of vitamin mixture resulted in the fact that animals received 20-23% of vitamins D, A, B2, 33% of vitamin B1 and 57% of vitamin E from the content of these vitamins in the diet of animals from control group because of some nature vitamins contained in such diet basic components as casein (vitamins D, A, B1, B2) and sunflower oil (vitamin E). After one month treatment a deep deficiency of all vitamins has developed in rats from this group. Liver level of vitamin A decreased 8,5-fold, vitamins E and B1 - approximately 2-fold, vitamins C and B2 by 22%. Urinary excretion of vitamin B1 and B2 declined 2 and 5,3 fold. Blood plasma concentration of vitamin A decreased 1,9 fold, and E - 1,4 fold, B2 - by 30%. Activities of blood plasma vitamin B6-dependent enzymes reduced 1,4-fold. 2-fold decrease in the amount of vitamin mixture ensured intake about 50-59% of vitamins D, A, B2, and B1 and about 71% of vitamin E of those contained in the diet of animals from control group. Vitamin status indexes of these animals occupied an intermediate position. They have developed a moderate deficit of these essential micronutrients. The proposed model may be useful for metabolic disorders identification, the study of vitamins and minerals assimilation, investigations of the influence of biologically active components of food on these processes, as well as the development of personalized approaches to the correction of vitamin insufficient accuracy.
提出了一种基于大鼠饮食中所有维生素部分同时减少的不同程度营养性多种维生素缺乏模型。该模型与其他实验模型有本质区别，其他模型基于从动物饮食中完全排除1种或2种维生素。所提出的模型能让你更接近实际观察到的人群中几种维生素的联合缺乏情况。维生素混合物减少5倍导致动物从对照组动物饮食中这些维生素的含量中获取了20 - 23%的维生素D、A、B2，33%的维生素B1和57%的维生素E，因为这种饮食的基本成分如酪蛋白（维生素D、A、B1、B2）和向日葵油（维生素E）中含有一些天然维生素。经过一个月的治疗，该组大鼠出现了所有维生素的严重缺乏。肝脏中维生素A的水平下降了8.5倍，维生素E和B1下降了约2倍，维生素C和B2下降了22%。维生素B1和B2的尿排泄量分别下降了2倍和5.3倍。血浆中维生素A的浓度下降了1.9倍，维生素E下降了1.4倍，维生素B2下降了30%。血浆中维生素B6依赖性酶的活性降低了1.4倍。维生素混合物量减少2倍确保动物摄入了对照组动物饮食中约50 - 59%的维生素D、A、B2和B1以及约71%的维生素E。这些动物的维生素状态指标处于中间位置。它们出现了这些必需微量营养素的中度缺乏。所提出的模型可能有助于识别代谢紊乱、研究维生素和矿物质的吸收、研究食物生物活性成分对这些过程的影响，以及开发个性化方法以更准确地纠正维生素不足。
