Nakaji Shinnosuke, Ueta Mayumi, Sotozono Chie, Inatomi Tsutomu, Kinoshita Shigeru
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Nippon Ganka Gakkai Zasshi. 2012 Jun;116(6):581-7.
Our previous study of polymorphisms in the HLA-class I genes of 71 Japanese SJS/TEN patients with ocular surface complications and 113 Japanese healthy controls showed that in the Japanese, HLA-A*0206 was strongly associated with SJS/ TEN. In this study, we examined 118 Japanese SJS/ TEN patients with ocular surface complications and a new control group consisting of 220 healthy Japanese volunteers, and investigated the association between HLA class I antigens, HLA-A, B, C, and the SJS/ TEN.
For HLA genotyping we enrolled 118 Japanese patients with SJS/TEN in the chronic or sub-acute phase at Kyoto Prefectural University of Medicine; all presented with ocular surface complications. We also enrolled 220 healthy Japanese volunteers. We performed polymerase chain reaction amplification followed by hybridization with sequence-specific oligonucleotide probes (PCR-SSO). Results : HLA-A*0206 was most strongly associated with Japanese SJS/TEN patients with ocular surface complications (carrier frequency: p = 0.0000000002, OR = 5.2; gene frequency : p = 0.000000007, OR = 4.2).
HLA-A* 0206 is strongly associated with Japanese SJS/TEN patients with ocular surface complications.
我们之前对71例患有眼表并发症的日本中毒性表皮坏死松解症/重症多形红斑（SJS/TEN）患者和113名日本健康对照者的HLA-Ⅰ类基因多态性进行的研究表明，在日本人中，HLA-A*0206与SJS/TEN密切相关。在本研究中，我们对118例患有眼表并发症的日本SJS/TEN患者以及由220名日本健康志愿者组成的新对照组进行了研究，并调查了HLA-Ⅰ类抗原、HLA-A、B、C与SJS/TEN之间的关联。
为进行HLA基因分型，我们纳入了京都府立医科大学处于慢性或亚急性期的118例日本SJS/TEN患者；所有患者均有眼表并发症。我们还纳入了220名日本健康志愿者。我们进行了聚合酶链反应扩增，随后与序列特异性寡核苷酸探针杂交（PCR-SSO）。结果：HLA-A*0206与患有眼表并发症的日本SJS/TEN患者关联最为密切（携带频率：p = 0.0000000002，比值比[OR]=5.2；基因频率：p = 0.000000007，OR = 4.2）。
HLA-A*0206与患有眼表并发症的日本SJS/TEN患者密切相关。
