Teng Xiao-Ming, Li Kun-Ming, Wang Yu, Pan Jia-Ping, Yin Ping, Liang Shan-Shan, Ruan Jing-Ling, Chen Zhi-Qin
Center of Reproductive Medicine, The First Maternity and Infant Hospital Affiliated to Tongji University School of Medicine, Shanghai 200040, China.
Zhonghua Nan Ke Xue. 2012 Jun;18(6):531-3.
To explore the feasibility, indication and method of oocyte vitrification during the IVF - ET procedure, so as to increase the utilization of oocytes and reduce oocyte waste.
This study included the patients whose husbands failed to provide sperm samples at the time of oocyte pickup or from whom more than 25 oocytes were obtained. With the patients' consent, some of their oocytes were subjected to cryopreservation by vitrification, and used for IVF - ET after thawed.
Totally, 53 oocytes from 7 patients were thawed, and 44 (83.02%) survived, of which 41 M II oocytes were subjected to ICSI and 32 (72.73%) were fertilized. Thirty embryos were formed, with a cleavage rate of 93.75%. Sixteen embryos were transferred in 9 cycles, with achievement of 2 clinical pregnancies and delivery of 3 healthy babies. The implantation rate was 18.75% and the live birth rate 22.22%. Seven of the embryos were still cryopreserved.
Cryopreservation of oocytes by vitrification effects satisfactory rates of survival and fertilization, and that of surplus oocytes can increase oocyte utilization and adds to the alternatives for IVF - ET.
探讨体外受精-胚胎移植（IVF-ET）过程中卵母细胞玻璃化冷冻的可行性、适应证及方法，以提高卵母细胞利用率，减少卵母细胞浪费。
本研究纳入取卵时丈夫未能提供精液样本或获取卵母细胞超过25枚的患者。经患者同意，将其部分卵母细胞进行玻璃化冷冻保存，解冻后用于IVF-ET。
共解冻7例患者的53枚卵母细胞，44枚（83.02%）存活，其中41枚MⅡ期卵母细胞行卵胞浆内单精子注射（ICSI），32枚（72.73%）受精。共形成30枚胚胎，卵裂率为93.75%。9个周期移植16枚胚胎，获得2例临床妊娠，分娩3名健康婴儿。种植率为18.75%，活产率为22.22%。7枚胚胎仍在冷冻保存。
卵母细胞玻璃化冷冻保存可获得满意的存活及受精率，冷冻多余卵母细胞可提高卵母细胞利用率，增加IVF-ET的选择。
