Ding Jianchi, Rana Nasir, Dmowski W Paul
Oak Brook Fertility Center, Oak Brook, IL 60523, USA.
J Reprod Med. 2008 Oct;53(10):813-20.
The technology for successful oocyte cryopreservation has evolved only over the past decade. Fewer than 300 pregnancies achieved with cryopreserved oocytes were reported as of the end of 2006. Two cryopreservation techniques are currently available: the slow-cooling method and vitrification. This article reviews the world literature on pregnancies conceived from cryopreserved oocytes and reports 3 such pregnancies conceived with a slow-cooling method in our facility.
Three patients who underwent in vitro fertilization/embryo transfer cycles had the supranumerary oocytes cryopreserved using a slow-cooling method. After a period of 2-7 months, the oocytes were thawed, fertilized and the resulting embryos were transferred. All 3 patients conceived and 2 delivered-1 singleton and 1 a set of twins. The third patient conceived twins and was in the third trimester of her pregnancy at this writing. Literature review indicates that the majority of pregnancies to date have been achieved with a slow-cooling protocol. However, recent advances in the vitrification technology have improved its effectiveness and because of its simplicity made it the method of choice in oocyte cryopreservation.
Both slow-freezing and vitrification methods for oocyte cryopreservation can be used effectively to achieve pregnancies. Vitrification is technically simpler and may be more effective than slow freezing.
成功的卵母细胞冷冻保存技术仅在过去十年中得到发展。截至2006年底，据报道通过冷冻保存的卵母细胞实现的妊娠少于300例。目前有两种冷冻保存技术：慢速冷冻法和玻璃化冷冻法。本文回顾了关于冷冻保存的卵母细胞受孕的世界文献，并报告了我们机构中3例通过慢速冷冻法受孕的此类妊娠。
3例接受体外受精/胚胎移植周期的患者使用慢速冷冻法冷冻保存了多余的卵母细胞。2至7个月后，将卵母细胞解冻、受精，并移植由此获得的胚胎。所有3例患者均受孕，其中2例分娩——1例单胎和1例双胞胎。第三位患者怀有双胞胎，在撰写本文时已处于妊娠晚期。文献综述表明，迄今为止大多数妊娠是通过慢速冷冻方案实现的。然而，玻璃化冷冻技术的最新进展提高了其有效性，并且由于其操作简单，使其成为卵母细胞冷冻保存的首选方法。
卵母细胞冷冻保存的慢速冷冻法和玻璃化冷冻法均可有效用于实现妊娠。玻璃化冷冻在技术上更简单，可能比慢速冷冻更有效。
