Hormones Department, Medical Research Division, National Research Centre, Cairo, Egypt.
Clin Biochem. 2012 Nov;45(16-17):1519-21. doi: 10.1016/j.clinbiochem.2012.06.032. Epub 2012 Jul 6.
This study aimed at exploring the role of curcumin and taurine alone or in combination against cultured human hepatoma cells (Huh-7 cells).
Huh-7 cells were plated and treated with various concentrations of curcumin and/or taurine. Hemocytometer cell count, cell viability, quantification of γ-IFN concentrations, and flow cytometric analyses for CD4, CD8, and CD25 were carried out.
There were significant increases in the levels of cell density, γ-IFN, and CD8, accompanied with significant decrease in the level of CD4, when comparing cultured cells treated with curcumin and taurine with control cultured cells.
Curcumin/taurine in combination formula is better treatment than single therapy, with respect to cell density and γ-IFN. Moreover, curcumin/taurine combined therapy enhances immunity by stimulating the CD4(+) T-helper cells with consequent induction of CD8 T-cell responses to lyse tumor cells.
本研究旨在探索姜黄素和牛磺酸单独或联合应用对培养的人肝癌细胞（Huh-7 细胞）的作用。
将 Huh-7 细胞接种并用不同浓度的姜黄素和/或牛磺酸处理。用血球计数器进行细胞计数，细胞活力测定，γ-IFN 浓度定量和 CD4、CD8 和 CD25 的流式细胞分析。
与对照培养细胞相比，用姜黄素和牛磺酸处理的培养细胞的细胞密度、γ-IFN 和 CD8 水平显著增加，而 CD4 水平显著降低。
与单一疗法相比，姜黄素/牛磺酸联合配方在细胞密度和 γ-IFN 方面是更好的治疗方法。此外，姜黄素/牛磺酸联合治疗通过刺激 CD4(+)辅助性 T 细胞，继而诱导 CD8 T 细胞对肿瘤细胞的溶解反应来增强免疫。
