盐酸阿比多尔对2009新型甲型流感病毒（H1N1）的体外作用
[The in-vitro effects of arbidol hydrochloride against 2009 new influenza virus A (H1N1)].
作者信息
Zhang Xing-quan, Dai Jun-peng, Cai Bai-qiang
机构信息
University of California, San Diego, CA 92093, USA.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2012 Apr;34(2):126-9. doi: 10.3881/j.issn.1000-503X.2012.02.005.
OBJECTIVE
To detect the in-vitro effects of arbidol hydrochloride against 2009 new influenza virus A (H1N1).
METHODS
The activity of arbidol hydrochloride against 2009 new influenza virus A (H1N1) was determined in MDCK cell cultures. Hemagglutination assay, observation of cytopathic effects, RT-PCR and quantitative RT-PCR tests were performed for determination of virus titers. Inhibition concentration 50% and cytotoxic concentration 50% were calculated with Chou's Menu of Dose-Effect Program.
RESULTS
Arbidol hydrochloride showed low cytotoxicity (cytotoxic concentration 50%>100 μmol/L)and significant anti-2009 new influenza virus A (H1N1) activity in cell cultures. Inhibition concentration 50% were (5.5 ± 0.9), (3.4 ± 0.8), and (1.5 ± 0.2) μmol/L in hemagglutination assay, cytopathic effect test, and quantitative RT-PCR assay, respectively.
CONCLUSION
Arbidol has low cytotoxicity and high anti-virus activity and can effectively trigger the activities of interferon and immune response, and therefore can be a valuable anti-influenza virus drug.
目的
检测盐酸阿比多尔对2009新型甲型流感病毒（H1N1）的体外作用。
方法
在MDCK细胞培养物中测定盐酸阿比多尔对2009新型甲型流感病毒（H1N1）的活性。采用血凝试验、细胞病变效应观察、RT-PCR和定量RT-PCR检测来测定病毒滴度。用Chou氏剂量效应程序计算50%抑制浓度和50%细胞毒性浓度。
结果
盐酸阿比多尔在细胞培养物中显示出低细胞毒性（50%细胞毒性浓度>100μmol/L）和显著的抗2009新型甲型流感病毒（H1N1）活性。在血凝试验、细胞病变效应试验和定量RT-PCR试验中，50%抑制浓度分别为（5.5±0.9）、（3.4±0.8）和（1.5±0.2）μmol/L。
结论
阿比多尔具有低细胞毒性和高抗病毒活性，能有效激发干扰素活性和免疫反应，因此可成为一种有价值的抗流感病毒药物。
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