Liu Chao, Min Su, Wei Ke, Liu Dong, Dong Jun, Luo Jie, Liu Xiao-bin
Department of Anesthesiology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2012 Jun;34(3):216-21. doi: 10.3881/j.issn.1000-503X.2012.03.005.
To explore the effect of the electroconvulsive shock (ECS) on the glutamate level and the hyperphosphorylation of tau protein in depressed rats.
The depression rat models whose olfactory bulbs were removed were established. Using the analysis of variance of factorial design, we set up two intervention factors including electric current (three levels: 25, 50, and 75 mA) and duration (three levels: 3, 6, and 9 times), which constituted 9 combinations (n=6). Fifty-four adult depression rat models whose olfactory bulbs were removed were randomly divided into nine experimental groups (n=6 in each group). The hippocampus was removed within 12 hours after the ECS finished. The level of glutamate in the hippocampus was detected by high-performance liquid chromatography, and that of Tau protein, which includes p-PHF-1(Ser396/404), p-AT8(Ser199/202), and p-12E8(Ser262), in the hippocampus with Western blot analysis.
The glutamate level and the hyperphosphorylation of tau protein in the hippocampus of depressed rats remarkably increased. The changes of the hyperphosphorylation of tau protein were correlated with the electric current and duration of ECS, and these two factors showed an synergic effect.
ECS enhances the hyperphosphorylation of tau protein in the hippocampus of depressed rats by up-regulating the glutamate level.
探讨电惊厥休克（ECS）对抑郁大鼠谷氨酸水平及tau蛋白过度磷酸化的影响。
建立切除嗅球的抑郁大鼠模型。采用析因设计方差分析，设置电流（三个水平：25、50和75 mA）和持续时间（三个水平：3、6和9次）两个干预因素，构成9种组合（n = 6）。将54只成年切除嗅球的抑郁大鼠模型随机分为9个实验组（每组n = 6）。在ECS结束后12小时内取出海马体。采用高效液相色谱法检测海马体中谷氨酸水平，并用蛋白质免疫印迹分析法检测海马体中Tau蛋白水平，包括p - PHF - 1(Ser396/404)、p - AT8(Ser199/202)和p - 12E8(Ser262)。
抑郁大鼠海马体中谷氨酸水平及tau蛋白过度磷酸化显著增加。tau蛋白过度磷酸化的变化与ECS的电流和持续时间相关，且这两个因素表现出协同作用。
ECS通过上调谷氨酸水平增强抑郁大鼠海马体中tau蛋白的过度磷酸化。
