University of Minho, Department of Textile Engineering, Campus de Azurém, Guimarães, Portugal.
Biotechnol J. 2012 Nov;7(11):1376-85. doi: 10.1002/biot.201200142. Epub 2012 Aug 1.
The use of active ingredients in wound management have evolved alongside the pharmaceutical agents and dressings used to deliver them. However, the development of gauzes, dressings with specific properties, still remains a challenge for several medical applications. A new methodology for the controlled release of active components for the healing of burn wounds is proposed herein. Cotton and non-woven bandages have been cationised to promote the attachment of protein microspheres. The active agents, piroxicam and vegetable oil, were entrapped into the microspheres using ultrasound energy. Active agents were released from the microspheres by a change in pH. Wound healing was assessed through the use of standardised burn wounds induced by a cautery in human full-thickness skin equivalents (EpidermFT). The best re-epithelialisation and fastest wound closure was observed in wounds treated with proteinaceous microspheres attached to gauzes, after six days of healing, in comparison with commercial collagen dressing and other controls. Furthermore, the ability of these materials to reduce the inflammation process, together with healing improvement, makes these biomaterials suitable for wound-dressing applications.
在伤口管理中使用活性成分的方法已经随着用于输送这些成分的药物制剂和敷料的发展而发展。然而，对于许多医疗应用来说，开发具有特定性能的纱布和敷料仍然是一个挑战。本文提出了一种用于烧伤伤口愈合的活性成分控制释放的新方法。棉和无纺绷带已被阳离子化以促进蛋白质微球的附着。使用超声能量将活性药物吡罗昔康和植物油包埋入微球中。通过 pH 值的变化，活性药物从微球中释放出来。通过使用在人全厚皮肤等效物（EpidermFT）上用烙器诱导的标准烧伤伤口来评估伤口愈合。与商业胶原蛋白敷料和其他对照相比，在愈合六天后，附着在绷带上的蛋白质微球处理的伤口表现出最佳的再上皮化和最快的伤口闭合。此外，这些材料减轻炎症过程的能力以及对愈合的改善，使得这些生物材料适用于伤口敷料应用。
