Mohandas Annapoorna, Kumar P T Sudheesh, Raja Biswas, Lakshmanan Vinoth-Kumar, Jayakumar Rangasamy
Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi, India.
Int J Nanomedicine. 2015 Oct 1;10 Suppl 1(Suppl 1):53-66. doi: 10.2147/IJN.S79981. eCollection 2015.
Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel. The developed composite bandage was porous with porosity at a range of 60%-70%. The swelling ratios of the bandages decreased with increasing concentrations of nZnO. The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO. The prepared composite bandages exhibited excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and methicillin resistant S. aureus (MRSA). Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations. The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages.
通过冷冻干燥法，由纳米氧化锌（nZnO）和海藻酸盐水凝胶的混合物制备了海藻酸盐水凝胶/纳米氧化锌（nZnO）复合绷带。所制备的复合绷带具有多孔结构，孔隙率在60%-70%范围内。绷带的溶胀率随nZnO浓度的增加而降低。与不含nZnO的KALTOSTAT®绷带和对照绷带相比，掺入nZnO的复合绷带显示出可控的降解曲线和更快的凝血能力。所制备的复合绷带对大肠杆菌、金黄色葡萄球菌、白色念珠菌和耐甲氧西林金黄色葡萄球菌（MRSA）表现出优异的抗菌活性。通过阿拉玛（Alamar）试验和浸润研究对所制备的复合绷带在人皮肤成纤维细胞上进行细胞相容性评估，结果证明在较低nZnO浓度下绷带具有无毒性质，而随着nZnO浓度的增加，细胞活力略有下降。对猪皮肤进行的体外再上皮化定性分析表明，nZnO海藻酸盐绷带的角质形成细胞向伤口区域浸润。
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