基因靶向和颜色转换荧光探针。
Genetically targetable and color-switching fluorescent probe.
机构信息
Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.
出版信息
Chembiochem. 2012 Jul 23;13(11):1564-8. doi: 10.1002/cbic.201200334. Epub 2012 Jul 6.
Abstract
Color bind: We have developed a probe TMR-para-MG that switches its fluorescence emission upon binding to a fluorogen-activating protein (FAP). In cells that express FAP, this dye labels target sites in one color and mitochondria in another color, thus it might be a suitable tool for monitoring changes in mitochondrial membrane potential.
摘要
颜色绑定
我们开发了一种探针 TMR-para-MG，它在与荧光素激活蛋白 (FAP) 结合时会改变其荧光发射。在表达 FAP 的细胞中，这种染料将靶标标记为一种颜色，线粒体标记为另一种颜色，因此它可能是监测线粒体膜电位变化的合适工具。
相似文献
1
Genetically targetable and color-switching fluorescent probe.基因靶向和颜色转换荧光探针。
Chembiochem. 2012 Jul 23;13(11):1564-8. doi: 10.1002/cbic.201200334. Epub 2012 Jul 6.
2
Protein Proximity Observed Using Fluorogen Activating Protein and Dye Activated by Proximal Anchoring (FAP-DAPA) System.使用荧光蛋白激活蛋白和通过邻近锚定激活的染料（FAP-DAPA）系统观察蛋白质接近。
ACS Chem Biol. 2020 Sep 18;15(9):2433-2443. doi: 10.1021/acschembio.0c00419. Epub 2020 Aug 17.
3
Near-Infrared Fluorescent Probes for Imaging of Intracellular Mg and Application to Multi-Color Imaging of Mg, ATP, and Mitochondrial Membrane Potential.用于细胞内镁成像的近红外荧光探针及其在镁、ATP 和线粒体膜电位的多色成像中的应用。
Anal Chem. 2020 Jan 7;92(1):966-974. doi: 10.1021/acs.analchem.9b03872. Epub 2019 Nov 27.
4
High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues.使用双光子 FRET 系统进行活细胞和组织中线粒体 pH 的高深度荧光成像。
Chem Commun (Camb). 2018 Dec 14;54(96):13531-13534. doi: 10.1039/c8cc07934j. Epub 2018 Nov 15.
5
Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells.用于缺氧的可逆开-关荧光探针及其在活细胞中缺氧-常氧循环成像。
J Am Chem Soc. 2012 Dec 5;134(48):19588-91. doi: 10.1021/ja310049d. Epub 2012 Nov 26.
6
Imaging Reversible Mitochondrial Membrane Potential Dynamics with a Masked Rhodamine Voltage Reporter.用掩蔽的罗丹明电压报告基因成像可逆的线粒体膜电位动力学。
J Am Chem Soc. 2021 Mar 24;143(11):4095-4099. doi: 10.1021/jacs.0c13110. Epub 2021 Mar 12.
7
A seminaphthorhodafluor-based near-infrared fluorescent probe for hydrazine and its bioimaging in living systems.基于半萘并[1,8,9- def]香二氮杂萘的近红外荧光探针用于水合肼及其在活细胞中的生物成像。
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Dec 5;223:117307. doi: 10.1016/j.saa.2019.117307. Epub 2019 Jun 22.
8
Localization microscopy using noncovalent fluorogen activation by genetically encoded fluorogen-activating proteins.基于基因编码的荧光蛋白非共价激活的定位显微镜。
Chemphyschem. 2014 Mar 17;15(4):687-695. doi: 10.1002/cphc.201300757. Epub 2013 Nov 5.
9
A tumor-specific and mitochondria-targeted fluorescent probe for real-time sensing of hypochlorite in living cells.一种用于实时检测活细胞中次氯酸盐的肿瘤特异性线粒体靶向荧光探针。
Chem Commun (Camb). 2017 May 17;53(40):5539-5541. doi: 10.1039/c7cc01679d.
10
Multiexcitation Fluorogenic Labeling of Surface, Intracellular, and Total Protein Pools in Living Cells.活细胞中表面、细胞内和总蛋白库的多激发荧光标记
Bioconjug Chem. 2016 Jun 15;27(6):1525-31. doi: 10.1021/acs.bioconjchem.6b00169. Epub 2016 May 19.
引用本文的文献
1
Oxidative Synthesis of Acid Blue 7 Dye Catalyzed by CuO/Silicotungstic Acid in Water-Phase.水相中CuO/硅钨酸催化氧化合成酸性蓝7染料
Materials (Basel). 2021 Aug 11;14(16):4505. doi: 10.3390/ma14164505.
2
Super-resolution Microscopy with Single Molecules in Biology and Beyond-Essentials, Current Trends, and Future Challenges.超越生物学的单分子超分辨率显微镜——要点、当前趋势和未来挑战。
J Am Chem Soc. 2020 Oct 21;142(42):17828-17844. doi: 10.1021/jacs.0c08178. Epub 2020 Oct 9.
3
Protein Proximity Observed Using Fluorogen Activating Protein and Dye Activated by Proximal Anchoring (FAP-DAPA) System.使用荧光蛋白激活蛋白和通过邻近锚定激活的染料（FAP-DAPA）系统观察蛋白质接近。
ACS Chem Biol. 2020 Sep 18;15(9):2433-2443. doi: 10.1021/acschembio.0c00419. Epub 2020 Aug 17.
4
Fluorogen-activating proteins: beyond classical fluorescent proteins.荧光团激活蛋白：超越传统荧光蛋白
Acta Pharm Sin B. 2018 May;8(3):339-348. doi: 10.1016/j.apsb.2018.02.001. Epub 2018 Mar 24.
5
A versatile optical tool for studying synaptic GABA receptor trafficking.一种用于研究突触GABA受体转运的多功能光学工具。
J Cell Sci. 2017 Nov 15;130(22):3933-3945. doi: 10.1242/jcs.205286. Epub 2017 Oct 12.
6
Breaking the color barrier - a multi-selective antibody reporter offers innovative strategies of fluorescence detection.突破肤色障碍——一种多选择性抗体报告分子提供了荧光检测的创新策略。
J Cell Sci. 2017 Aug 1;130(15):2644-2653. doi: 10.1242/jcs.202952. Epub 2017 Jun 14.
7
Multiexcitation Fluorogenic Labeling of Surface, Intracellular, and Total Protein Pools in Living Cells.活细胞中表面、细胞内和总蛋白库的多激发荧光标记
Bioconjug Chem. 2016 Jun 15;27(6):1525-31. doi: 10.1021/acs.bioconjchem.6b00169. Epub 2016 May 19.
8
Genetically targeted fluorogenic macromolecules for subcellular imaging and cellular perturbation.用于亚细胞成像和细胞扰动的基因靶向荧光大分子。
Biomaterials. 2015 Oct;66:1-8. doi: 10.1016/j.biomaterials.2015.07.002. Epub 2015 Jul 2.
9
Engineering tandem single-chain Fv as cell surface reporters with enhanced properties of fluorescence detection.工程化串联单链抗体片段作为具有增强荧光检测特性的细胞表面报告分子。
Protein Eng Des Sel. 2015 Oct;28(10):327-37. doi: 10.1093/protein/gzv016. Epub 2015 Apr 5.
10
Advances in chemical labeling of proteins in living cells.活细胞中蛋白质化学标记的进展。
Cell Tissue Res. 2015 Apr;360(1):179-94. doi: 10.1007/s00441-015-2145-4. Epub 2015 Mar 7.
本文引用的文献
1
A caged, localizable rhodamine derivative for superresolution microscopy.一种笼状、可定位的若丹明衍生物，用于超分辨率显微镜。
ACS Chem Biol. 2012 Feb 17;7(2):289-93. doi: 10.1021/cb2002889. Epub 2011 Nov 4.
2
An expanded palette of genetically encoded Ca²⁺ indicators.基因编码 Ca²⁺指示剂的扩展库。
Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592. Epub 2011 Sep 8.
3
Germ-layer and lineage-restricted stem/progenitors regenerate the mouse digit tip.胚层和谱系限制的干细胞/祖细胞可使小鼠指尖再生。
Nature. 2011 Aug 24;476(7361):409-13. doi: 10.1038/nature10346.
4
Principles for designing fluorescent sensors and reporters.荧光传感器和报告分子的设计原则。
Nat Chem Biol. 2011 Jul 18;7(8):480-3. doi: 10.1038/nchembio.620.
5
Electrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein.利用荧光电压指示蛋白探测大肠杆菌中的电尖峰。
Science. 2011 Jul 15;333(6040):345-8. doi: 10.1126/science.1204763.
6
Labeling mitochondria with TMRM or TMRE.用四甲基罗丹明甲酯（TMRM）或四甲基罗丹明乙酯（TMRE）标记线粒体。
Cold Spring Harb Protoc. 2011 Jul 1;2011(7):895-7. doi: 10.1101/pdb.prot5641.
7
Oxytocin selectively gates fear responses through distinct outputs from the central amygdala.催产素通过中杏仁核的不同输出选择性地调节恐惧反应。
Science. 2011 Jul 1;333(6038):104-7. doi: 10.1126/science.1201043.
8
Real-time observation of transcription initiation and elongation on an endogenous yeast gene.真核基因转录起始和延伸的实时观察
Science. 2011 Apr 22;332(6028):475-8. doi: 10.1126/science.1202142.
9
Reporting from the field: genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems.现场报道：基因编码荧光报告蛋白揭示活体生物系统中的信号动态。
Annu Rev Biochem. 2011;80:375-401. doi: 10.1146/annurev-biochem-060409-093259.
10
Genetically encoded copper-free click chemistry.基因编码的无铜点击化学
Angew Chem Int Ed Engl. 2011 Apr 18;50(17):3878-81. doi: 10.1002/anie.201008178. Epub 2011 Mar 23.
Zotero 插件