Khazaei Majid, Barmaki Babak
Department of Physiology, Isfahan University of Medical Sciences, Isfahan 81746-73836, Iran.
J Biomed Biotechnol. 2012;2012:365195. doi: 10.1155/2012/365195. Epub 2012 Jun 12.
In this study, we investigated the role of exogenous NO donor, sodium nitroprusside (SNP), on hemodynamic responses and survival rate during decompensated hemorrhagic shock in normotensive and hypertensive rat.
Male wistar rats were divided into normotensive and hypertensive groups (n = 12 each). Then, the animals were subjected to decompensated hemorrhagic shock by withdrawing blood until the mean arterial pressure (MAP) reached to 40 mmHg. After the shock period, the animals were randomly assigned to SNP-treated (0.5 mg/kg) and control groups (n = 6 each). MAP and heart rate (HR) were monitored throughout the experiment and 60 min after the administration of drug. Serum NO concentrations were measured. The survival rate was counted during next 72 h.
Infusion of SNP caused no significant changes in MAP and HR in normotensive and hypertensive animals. Hemorrhagic shock increased serum NO concentration and SNP administration reduced serum NO concentration in either normotensive or hypertensive groups. Survival counts during 72 h after experiment did not improve by SNP administration, and there were no significant differences between normotensive and hypertensive groups.
SNP administration cannot improve hemodynamic responses and survival count during decompensated hemorrhagic shock in normotensive and hypertensive animals.
在本研究中，我们调查了外源性一氧化氮供体硝普钠（SNP）在正常血压和高血压大鼠失代偿性出血性休克期间对血流动力学反应和存活率的作用。
雄性Wistar大鼠分为正常血压组和高血压组（每组n = 12）。然后，通过放血使动物遭受失代偿性出血性休克，直至平均动脉压（MAP）达到40 mmHg。休克期过后，将动物随机分为SNP治疗组（0.5 mg/kg）和对照组（每组n = 6）。在整个实验过程以及给药后60分钟监测MAP和心率（HR）。测量血清一氧化氮浓度。在接下来的72小时内计算存活率。
在正常血压和高血压动物中，输注SNP对MAP和HR无显著影响。失血性休克增加了血清一氧化氮浓度，而在正常血压或高血压组中，给予SNP均降低了血清一氧化氮浓度。给予SNP并未改善实验后72小时内的存活计数，正常血压组和高血压组之间也无显著差异。
在正常血压和高血压动物的失代偿性出血性休克期间，给予SNP不能改善血流动力学反应和存活计数。
