EPFL-École Polytechnique Fédérale de Lausanne, MXG 321, Station 12, Lausanne CH-1015, Switzerland.
Sensors (Basel). 2012;12(5):6520-37. doi: 10.3390/s120506520. Epub 2012 May 18.
We report on the electrochemical detection of anti-cancer drugs in human serum with sensitivity values in the range of 8-925 nA/μM. Multi-walled carbon nanotubes were functionalized with three different cytochrome P450 isoforms (CYP1A2, CYP2B6, and CYP3A4). A model used to effectively describe the cytochrome P450 deposition onto carbon nanotubes was confirmed by Monte Carlo simulations. Voltammetric measurements were performed in phosphate buffer saline (PBS) as well as in human serum, giving well-defined current responses upon addition of increasing concentrations of anti-cancer drugs. The results assert the capability to measure concentration of drugs in the pharmacological ranges in human serum. Another important result is the possibility to detect pairs of drugs present in the same sample, which is highly required in case of therapies with high side-effects risk and in anti-cancer pharmacological treatments based on mixtures of different drugs. Our technology holds potentials for inexpensive multi-panel drug-monitoring in personalized therapy.
我们报告了在人血清中电化学检测抗癌药物的方法，其灵敏度值在 8-925 nA/μM 范围内。多壁碳纳米管通过三种不同的细胞色素 P450 同工酶（CYP1A2、CYP2B6 和 CYP3A4）进行了功能化。通过蒙特卡罗模拟证实了一种可有效描述细胞色素 P450 沉积到碳纳米管上的模型。在磷酸盐缓冲盐水 (PBS) 以及人血清中进行了伏安测量，在加入浓度不断增加的抗癌药物时，会产生明确的电流响应。结果证明了在人血清中测量药理范围内药物浓度的能力。另一个重要的结果是有可能检测同一样品中存在的一对药物，这在副作用风险高的治疗和基于不同药物混合物的抗癌药物治疗中非常需要。我们的技术具有在个性化治疗中进行廉价的多面板药物监测的潜力。
