School of Pre-Clinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Evid Based Complement Alternat Med. 2012;2012:154271. doi: 10.1155/2012/154271. Epub 2012 Jun 24.
Norcantharidin (NCTD) is the demethylated form of cantharidin, which is the active substance of mylabris, and is known to have anticancer potentials. The aim of this paper was to assess the apoptosis-inducing effect of NCTD on HL-60 cells. Methods. The effects of NCTD were detected by flow cytometer on the cell toxicity, cell cycle, and apoptosis of HL-60 cells cultured in vitro. Results. After 48-hour treatment with NCTD, the growth of HL-60 cells was inhibited significantly. The summit of apoptosis appeared after 24 hours. The percentage of the cells in G(1) phase decreased and then increased in S and G(2)+ M phase, while the S and G(2)+ M phases were blocked after treatment with 5, 10, and 50 μmol/L NCTD for 24 hours. Conclusions. NCTD can induce the apoptosis of HL-60 cells and inhibit the fissiparism, and the domino effect was obviously correlated with the time and dosage.
去甲斑蝥素（NCTD）是斑蝥素的去甲基化形式，是斑蝥的活性物质，已知具有抗癌潜力。本文旨在评估 NCTD 对 HL-60 细胞的诱导凋亡作用。方法。通过流式细胞仪检测 NCTD 对体外培养的 HL-60 细胞的细胞毒性、细胞周期和凋亡的影响。结果。NCTD 处理 48 小时后，HL-60 细胞的生长明显受到抑制。凋亡的高峰出现在 24 小时后。细胞在 G1 期的百分比减少，然后在 S 和 G2+M 期增加，而用 5、10 和 50μmol/L NCTD 处理 24 小时后，S 和 G2+M 期被阻断。结论。NCTD 可诱导 HL-60 细胞凋亡，抑制分裂，多米诺骨牌效应与时间和剂量明显相关。
