Ye Kun, Wei Qiaoyu, Gong Zhifeng, Huang Yunfeng, Liu Hong, Li Ying, Peng Xiaomei
a Department of Nephrology , The People's Hospital of Guangxi Zhuang Autonomous Region , Guangxi , China.
b Department of Nephrology , The Second Xiangya Hospital of Central South University , Hunan , China.
Ren Fail. 2017 Nov;39(1):458-464. doi: 10.1080/0886022X.2017.1308257.
Norcantharidin (NCTD) regulates immune system function and reduces proteinuria. We sought to investigate the effect of NCTD on proliferation, apoptosis and cell cycle of cultured human mesangial cells (HMC) in vitro.
HMC cells were divided into a normal control group, and various concentrations of NCTD group (2.5, 5, 10, 20, or 40 μg/mL). Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, apoptosis was detected by Annexin V/propidium iodide (PI) assays, and morphological analysis was performed by Hoechest 33258 staining. Finally, cell cycle was analyzed by flow cytometry.
NCTD dose and time dependently inhibits HMC proliferation significantly (p < .05). Apoptosis dose and time dependently increased after NCTD treatment. Cell-cycle analysis revealed that the number of cells in the G2 phase increased significantly, whereas the fraction of cells in the S phase decreased, especially 24 h after 5 μg/ml NCTD treatment.
NCTD inhibits HMC cell proliferation, induces apoptosis, and affects the cell cycle.
去甲斑蝥素（NCTD）可调节免疫系统功能并减少蛋白尿。我们试图研究NCTD对体外培养的人系膜细胞（HMC）增殖、凋亡和细胞周期的影响。
将HMC细胞分为正常对照组和不同浓度的NCTD组（2.5、5、10、20或40μg/mL）。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法测定细胞增殖，采用膜联蛋白V/碘化丙啶（PI）法检测凋亡，采用Hoechest 33258染色进行形态学分析。最后，通过流式细胞术分析细胞周期。
NCTD剂量和时间依赖性地显著抑制HMC增殖（p<0.05）。NCTD处理后凋亡呈剂量和时间依赖性增加。细胞周期分析显示，G2期细胞数量显著增加，而S期细胞比例下降，尤其是在5μg/ml NCTD处理24小时后。
NCTD抑制HMC细胞增殖，诱导凋亡，并影响细胞周期。
