[高效液相色谱法测定人参中十二种人参皂苷]
[Determination of twelve ginsenosides in Panax ginseng by HPLC].
作者信息
Xu Xiuying, Zheng Yimin, Fu Shanquan, Zhao Ying, Li Jie, Wang Linlin
机构信息
College of Pharmaceutical and Biological Engineering, Chongqing University of Technology, Chongqing 400054, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Jun;36(11):1463-5.
OBJECTIVE
To determine the contents of twelve ginsenosides in the root of Panax ginseng by HPLC.
METHOD
The analysis is carried out at room temperature on a Luna NH2 column (4.6 mm x 150 mm, 5 microm) eluted with acetonitrile and water as the mobile phases in a gradient elution. The flow-rate was 1.0 mL x min(-1), the detection wavelength was 203 nm.
RESULT
Twelve ginsenosides (Rh2, Rh1, Rg2, Rg3, Rg1, Rf, Re, Rd, Re, Rb2, Rb3, Rb1) were separated at baseline within 60 min with good linearity (r > or = 0.999 5). The recovery rates were 98.1%, 95.3%, 96.1%, 95.6%, 97.3%, 98.6%, 98.0%, 96.4%, 96.1%, 97.6%, 96.8%, 96.9% (RSD < or = 3.0%).
CONCLUSION
The method was simple,fast and could control the quality of P. ginseng effectively.
目的
采用高效液相色谱法测定人参根中12种人参皂苷的含量。
方法
在室温下，于Luna NH2柱（4.6 mm×150 mm，5μm）上进行分析，以乙腈和水为流动相进行梯度洗脱。流速为1.0 mL·min⁻¹，检测波长为203 nm。
结果
12种人参皂苷（Rh2、Rh1、Rg2、Rg3、Rg1、Rf、Re、Rd、Rc、Rb2、Rb3、Rb1）在60分钟内实现基线分离，线性关系良好（r≥0.999 5）。回收率分别为98.1%、95.3%、96.1%、95.6%、97.3%、98.6%、98.0%、96.4%、96.1%、97.6%、96.8%、96.9%（相对标准偏差≤3.0%）。
结论
该方法简便、快速，可有效控制人参的质量。
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