Li Jie, Jiang Juan, Zheng Yimin, Wang Linlin, Yang Yuqing, Hu Yang
College of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400050, China.
Zhongguo Zhong Yao Za Zhi. 2011 Sep;36(17):2380-2.
To establish an HPLC method for simultaneous determination of panaxynol and panaxydol from the fibrous root of Panax ginseng.
The analysis was performed on Elite C18 column (4.6 mm x 150 mm, 5 microm) with mobile phase gradient of CH3CN-water at a flow rate of 1.0 mL x min(-1). The detection wavelength was 230 nm, and the detection temperature was ambient.
The linear range were 0.70-3.50 microg (r = 0.9995) for panaxynol, and 0.64-3.20 microg (r = 0.9999) for panaxydol. The average recoveries were 99.1% (RSD 1.7%) and 99.3% (RSD 1.2%), respectively.
The HPLC method is simple, rapid and reproducible, which can be used for the quality control of the fibrous root of P. ginseng.
建立同时测定人参须根中人参炔醇和人参二醇的高效液相色谱法。
采用Elite C18柱（4.6 mm×150 mm，5μm），以乙腈-水为流动相进行梯度洗脱，流速为1.0 mL·min⁻¹。检测波长为230 nm，检测温度为室温。
人参炔醇的线性范围为0.70 - 3.50μg（r = 0.9995），人参二醇的线性范围为0.64 - 3.20μg（r = 0.9999）。平均回收率分别为99.1%（RSD 1.7%）和99.3%（RSD 1.2%）。
该高效液相色谱法简便、快速、重现性好，可用于人参须根的质量控制。
