体外抗弓形虫活性的根提取物/ fractions 的 Eurycoma longifolia Jack。
In vitro anti-Toxoplasma gondii activity of root extract/fractions of Eurycoma longifolia Jack.
机构信息
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Pulau Pinang, Malaysia.
出版信息
BMC Complement Altern Med. 2012 Jul 10;12:91. doi: 10.1186/1472-6882-12-91.
BACKGROUND
Toxoplasma gondii infection causes toxoplasmosis, an infectious disease with worldwide prevalence. The limited efficiency of drugs against this infection, their side effects and the potential appearance of resistant strains make the search of novel drugs an essential need. We examined Eurycoma longifolia root extract and fractions as potential sources of new compounds with high activity and low toxicity. The main goal of this study was to investigate the anti-T. gondii activity of crude extract (TACME) and four fractions (TAF 273, TAF 355, TAF 191 and TAF 401) from E. longifolia, with clindamycin as the positive control.
METHODS
In vitro toxoplasmacidal evaluation was performed using Vero cells as host for T. gondii. Light microscopy technique was used to study in situ antiparasitic activity.
RESULTS
Significant anti-T. gondii activity was observed with clindamycin (EC50 = 0.016 μg/ml), follow by TAF 355 (EC50 = 0.369 μg/ml) and TAF 401 (EC50 = 0.882 μg/ml). Light microscopy revealed that most Vero cells were infected after 3 h of exposure to T. gondii. After 36 h of exposure to the E. longifolia fraction, the host Vero cells showed no visible intracellular parasite and no remarkable morphological changes.
CONCLUSIONS
Our study demonstrated that TAF 355 and TAF401 fractions may be the sources of new anti-T. gondii compounds.
背景
刚地弓形虫感染引起弓形体病，这是一种在世界范围内流行的传染病。目前针对这种感染的药物疗效有限，存在副作用，且可能出现耐药株，因此寻找新型药物成为当务之急。我们研究了长叶黄杞根提取物及其 4 个馏分（TAF273、TAF355、TAF191 和 TAF401），作为具有高活性和低毒性的新型化合物的潜在来源。本研究的主要目的是研究长叶黄杞根粗提取物（TACME）和 4 个馏分（TAF273、TAF355、TAF191 和 TAF401）对刚地弓形虫的抗弓形虫活性，克林霉素为阳性对照。
方法
使用 Vero 细胞作为刚地弓形虫的宿主，进行体外杀弓形虫活性评价。采用光学显微镜技术研究抗弓形虫的体内活性。
结果
克林霉素（EC50=0.016 μg/ml）显示出显著的抗刚地弓形虫活性，其次是 TAF355（EC50=0.369 μg/ml）和 TAF401（EC50=0.882 μg/ml）。光学显微镜显示，大多数 Vero 细胞在暴露于刚地弓形虫 3 小时后被感染。在接触长叶黄杞根馏分 36 小时后，宿主 Vero 细胞中没有可见的内寄生虫，且没有明显的形态变化。
结论
我们的研究表明，TAF355 和 TAF401 馏分可能是新的抗刚地弓形虫化合物的来源。
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