在双链 DNA 中直接监测损伤和修复的模板化学。
Templated chemistry for monitoring damage and repair directly in duplex DNA.
机构信息
Department of Chemistry, Stanford University, Stanford, California 94305, USA.
出版信息
Chem Commun (Camb). 2012 Aug 21;48(65):8069-71. doi: 10.1039/c2cc34060g. Epub 2012 Jul 10.
Abstract
We report the fluorogenic detection of the product of base excision repair (an abasic site) in a specific sequence of duplex DNA. This is achieved by DNA-templated chemistry, employing triple helix-forming probes that contain unnatural nucleobases designed to selectively recognize the site of a missing base. Light-up signals of up to 36-fold were documented, and probes could be used to monitor enzymatic removal of a damaged base.
摘要
我们报告了在双链 DNA 的特定序列中碱基切除修复（无碱基位点）产物的荧光检测。这是通过 DNA 模板化学实现的，使用了包含非天然碱基的三链形成探针，这些探针旨在选择性地识别缺失碱基的位点。记录到高达 36 倍的点亮信号，并且可以使用探针来监测酶去除受损碱基。
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