State Key Laboratory of Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China.
Arch Virol. 2012 Nov;157(11):2213-7. doi: 10.1007/s00705-012-1376-x. Epub 2012 Jul 11.
Cucumber mosaic virus (CMV) is one of the most devastating threats to the banana industry. A single-tube, one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of CMV-infected banana and plantain (Musa spp.). The reaction was performed in a single tube at 63 °C for 90 min using a real-time turbidimeter, with an improved closed-tube visual detection system in which fluorescent dye was added to the inside of the lid prior to amplification. This RT-LAMP assay is an alternative method for the rapid detection of CMV in banana plants and tissue culture materials.
黄瓜花叶病毒（CMV）是香蕉产业最具破坏性的威胁之一。本研究开发了一种单管一步逆转录环介导等温扩增（RT-LAMP）检测方法，用于快速检测感染 CMV 的香蕉和大蕉（Musa spp.）。该反应在 63°C 下使用实时浊度计在单个管中进行 90 分钟，在改进的封闭管目视检测系统中，在扩增前将荧光染料添加到盖子内部。该 RT-LAMP 检测方法是香蕉植物和组织培养材料中快速检测 CMV 的替代方法。
