Panno Stefano, Matić Slavica, Tiberini Antonio, Caruso Andrea Giovanni, Bella Patrizia, Torta Livio, Stassi Raffaele, Davino And Salvatore
Department of Agricultural, Food and Forest Sciences, University of Palermo, 90128 Palermo, Italy.
Department of Agricultural, Forestry and Food Sciences, University of Turin, 10095 Turin, Italy.
Plants (Basel). 2020 Apr 6;9(4):461. doi: 10.3390/plants9040461.
In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique. In this review, the authors provide an overview reporting in detail the different LAMP steps, focusing on designing and main characteristics of the primer set, different methods of result visualization, evolution and different application fields, reporting in detail LAMP application in plant virology, and the main advantages of the use of this technique.
在过去几十年中，分子诊断方法的发展产生了不同的先进工具，如环介导等温扩增技术（LAMP）。目前，由于其操作简单、特异性强、速度快且成本低，它已成为一种成熟的技术，应用于医学、农业和食品工业等不同领域。LAMP是一种等温条件下的核酸扩增技术，与即时检测（POC）分析高度兼容，具有改善植物保护诊断的潜力。LAMP的巨大优势促使其进行了多次升级以完善该技术。在这篇综述中，作者详细介绍了LAMP的不同步骤，重点阐述了引物组的设计和主要特点、结果可视化的不同方法、技术的发展及不同应用领域，详细报道了LAMP在植物病毒学中的应用以及使用该技术的主要优势。
