Imaizumi T
Department of Ophthalmology, Iwate Medical University, Morioka, Japan.
Nippon Ganka Gakkai Zasshi. 1990 Oct;94(10):928-36.
There is a hypothesis that corneal endothelial cells migrate after penetrating keratoplasty from the areas of higher cell density to the lower cell density areas. In order to confirm this hypothesis, the author performed exchange penetrating keratoplasty in normal albino rabbits using four combinations of sex and age and examined the movement of the endothelium between donors and recipients by observing the density and sex chromatin of the endothelial cells. As a result, there was no significant difference of the rate of decrease of the endothelial cell density of the postoperative grafts between young and old grafts. In the experiment using the sex chromatin of the endothelial cells as a cell marker, it was concluded that the endothelium of the rabbit cornea migrate from the young grafts to the old recipients or from the young recipients to the old grafts. In the control study exchanging rabbit grafts between animals of different sex but the same age the count of the sex chromatin of the endothelium of the grafts and recipients did not change postoperatively. The results confirmed the above hypothesis concerning the migration of the transplanted corneal endothelial cells in the rabbit cornea.
有一种假说认为，穿透性角膜移植术后角膜内皮细胞会从细胞密度较高的区域迁移至细胞密度较低的区域。为了证实这一假说，作者使用性别和年龄的四种组合，对正常白化兔进行了交换穿透性角膜移植，并通过观察内皮细胞的密度和性染色质，研究了供体和受体之间内皮细胞的移动情况。结果显示，术后移植物中，年轻移植物与年老移植物的内皮细胞密度下降速率无显著差异。在使用内皮细胞性染色质作为细胞标记的实验中，得出的结论是，兔角膜内皮细胞会从年轻移植物迁移至年老受体，或从年轻受体迁移至年老移植物。在一项对照研究中，将不同性别但年龄相同的兔子移植物进行交换，术后移植物和受体的内皮细胞性染色质计数未发生变化。这些结果证实了上述关于兔角膜中移植角膜内皮细胞迁移的假说。
