Bialasiewicz Seweryn, Mackay Ian M, Whiley David M, Sloots Theo P
Sir Albert Sakzewski Virus Research Centre, Herston, QLD, Australia.
Methods Mol Biol. 2012;903:225-33. doi: 10.1007/978-1-61779-937-2_14.
Etiologic agents of genital ulcer disease include herpes simplex 1 and 2, Treponema pallidum pallidum, Haemophilus ducreyi, and Klebsiella granulomatis. The advent of PCR has allowed for more rapid and sensitive detection of microbial pathogens. In this protocol, we describe the simultaneous detection of these five pathogens and an internal control using a single-tube multiplex PCR and colorimetric enzyme-linked amplicon hybridization assay.
生殖器溃疡疾病的病原体包括单纯疱疹病毒1型和2型、梅毒螺旋体、杜克雷嗜血杆菌和肉芽肿荚膜杆菌。聚合酶链反应（PCR）的出现使得对微生物病原体的检测更加快速和灵敏。在本方案中，我们描述了使用单管多重PCR和比色酶联扩增子杂交检测法同时检测这五种病原体及一种内部对照。
