ω-3 多不饱和脂肪酸促进大鼠 90%肝切除术后的肝再生。
Omega-3 polyunsaturated fatty acids promote liver regeneration after 90% hepatectomy in rats.
机构信息
Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital, Medical College of Nanjing University, Jiangsu Province's Key Medical Center for Hepatobiliary Disease, Nanjing 210008, Jiangsu Province, China.
出版信息
World J Gastroenterol. 2012 Jul 7;18(25):3288-95. doi: 10.3748/wjg.v18.i25.3288.
AIM
To evaluate the effectiveness of omega-3 polyunsaturated fatty acid (ω-3 PUFA) administration on liver regeneration after 90% partial hepatectomy (PH) in rats.
METHODS
ω-3 PUFAs were intravenously injected in the ω-3 PUFA group before PH surgery. PH, sparing only the caudate lobe, was performed in both the control and the ω-3 PUFA group. Survival rates, liver weight/body weight ratios, liver weights, HE staining, transmission electron microscope imaging, nuclear-associated antigen Ki-67, enzyme-linked immunosorbent assay and signal transduction were evaluated to analyze liver regeneration.
RESULTS
All rats in the control group died within 30 h after hepatectomy. Survival rates in the ω-3 PUFA group were 20/20 at 30 h and 4/20 1 wk after PH. Liver weight/body weight ratios and liver weights increased significantly in the ω-3 PUFA group. The structure of sinusoidal endothelial cells and space of Disse was greatly restored in the ω-3 PUFA group compared to the control group after PH. In the ω-3 PUFA group, interleukin (IL)-4 and IL-10 levels were significantly increased whereas IL-6 and tumor necrosis factor-α levels were dramatically decreased. In addition, activation of protein kinase B (Akt) and of signal transducer and activator of transcription 3 signaling pathway were identified at an earlier time after PH in the ω-3 PUFA group.
CONCLUSION
Omega-3 polyunsaturated fatty acids may prevent acute liver failure and promote liver regeneration after 90% hepatectomy in rats.
目的
评估ω-3 多不饱和脂肪酸（ω-3 PUFA）在大鼠 90%部分肝切除（PH）后对肝脏再生的有效性。
方法
在 PH 手术前，ω-3 PUFA 组通过静脉注射 ω-3 PUFA。在对照组和 ω-3 PUFA 组中，仅行尾状叶肝切除术。通过生存率、肝重/体重比、肝重、HE 染色、透射电镜成像、核相关抗原 Ki-67、酶联免疫吸附试验和信号转导来评估肝脏再生。
结果
对照组所有大鼠在 PH 后 30 h 内全部死亡。ω-3 PUFA 组在 PH 后 30 h 的生存率为 20/20，1 周后为 4/20。ω-3 PUFA 组的肝重/体重比和肝重显著增加。与对照组相比，PH 后 ω-3 PUFA 组的窦内皮细胞结构和 Disse 间隙得到了很大的恢复。在 ω-3 PUFA 组中，白细胞介素（IL）-4 和 IL-10 水平显著增加，而 IL-6 和肿瘤坏死因子-α水平显著降低。此外，在 PH 后更早时间ω-3 PUFA 组中发现蛋白激酶 B（Akt）和信号转导和转录激活因子 3 信号通路的激活。
结论
ω-3 多不饱和脂肪酸可预防大鼠 90%肝切除术后急性肝功能衰竭并促进肝脏再生。
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