Kumar Niranjan, Singh K P
Department of Crop Protection, Faculty of Agriculture, Ibrahim Badamasi Babangida University, Lapai, Niger State 066, Nigeria.
Mycobiology. 2011 Jun;39(2):113-7. doi: 10.4489/MYCO.2011.39.2.113. Epub 2011 Jun 16.
A laboratory experiment was conducted to study the induction of constricting rings and test predation of Dactylaria brochopaga isolates against second stage juveniles (J2s) of Meloidogyne graminicola. Among the five fungal isolates, isolate D showed the greatest number of predatory rings and, consequently, trapped the maximum number of M. graminicola J2s in dual cultures. Another pot experiment was conducted to study the effect of D. brochopaga (isolate D) on the management of wheat root-knot disease. Applying a mass culture (10 g/pot) and a spore suspension of the fungus with and without cow dung manure to soil infested with 2,000 M. graminicola juveniles significantly improved plant height, root length, weights of shoots, roots, panicles and grains per hill compared to those in the control. Moreover, the fungus significantly reduced the number of root-knots, the number of egg masses, juveniles, and females per hill compared to those in the control. Bio-efficacy of the fungus was heightened when the mass culture and a spore suspensions were used in combination with cow dung manure to improve the plant growth parameters and reduce the number of root-knot and reproductive factors. Further investigations should be conducted to identify the impact of this fungus in the field.
进行了一项实验室实验，以研究缢缩环的诱导情况，并测试布氏指梗霉分离株对禾谷根结线虫二期幼虫（J2s）的捕食作用。在五个真菌分离株中，分离株D形成的捕食环数量最多，因此，在共培养中捕获的禾谷根结线虫J2s数量也最多。还进行了另一项盆栽实验，以研究布氏指梗霉（分离株D）对小麦根结病防治的效果。在感染了2000条禾谷根结线虫幼虫的土壤中，施加真菌的大量培养物（10克/盆）和孢子悬浮液，无论是否添加牛粪，与对照相比，均显著提高了株高、根长、每穴地上部分、根系、穗和籽粒的重量。此外，与对照相比，该真菌显著减少了每穴根结、卵块、幼虫和雌虫的数量。当大量培养物和孢子悬浮液与牛粪一起使用时，真菌的生物防治效果增强，从而改善了植株生长参数，并减少了根结数量和繁殖因子。应进一步开展调查，以确定这种真菌在田间的影响。
