Zhang N, Zhang J, Wang Z W, Zha L, Huang Z
Department of General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.
Oncol Lett. 2012 May;3(5):1017-1022. doi: 10.3892/ol.2012.619. Epub 2012 Feb 28.
The effects of the interaction between KLF4 and β-catenin may be significant in human carcinogenesis and tumor development. This study aimed to determine whether the expression of KLF4 and β-catenin in gastric cancer tissues is associated with clinicopathological characteristics. Western blot analysis and immunohistochemistry were performed to detect KLF4 and β-catenin expression in tumor and corresponding non-cancerous tissues from 49 patients. The data revealed that KLF4 expression was significantly reduced in gastric cancer tissues compared with normal tissues. By contrast, the expression of the β-catenin protein was significantly increased in all tumor tissues, but was not expressed in distant normal mucosae. The altered expression of the KLF4 and β-catenin proteins was associated with advanced tumor stage and gastric cancer. In addition, the expression of the KLF4 and β-catenin proteins was inversely associated in moderately differentiated human gastric cancers. This study showed that β-catenin expression is significantly increased and KLF4 protein expression is markedly decreased in gastric cancer tissues, thus showing that the expression of KLF4 is inversely correlated with that of β-catenin in gastric cancer. The altered expression of the two proteins is associated with advanced tumor stage in gastric cancer.
KLF4与β-连环蛋白之间的相互作用在人类致癌作用和肿瘤发展中可能具有重要意义。本研究旨在确定KLF4和β-连环蛋白在胃癌组织中的表达是否与临床病理特征相关。采用蛋白质免疫印迹分析和免疫组织化学方法检测了49例患者肿瘤组织及相应癌旁组织中KLF4和β-连环蛋白的表达。数据显示，与正常组织相比，胃癌组织中KLF4表达显著降低。相比之下，β-连环蛋白在所有肿瘤组织中的表达均显著增加，但在远处正常黏膜中未表达。KLF4和β-连环蛋白的表达改变与肿瘤晚期和胃癌有关。此外，在中分化的人胃癌中，KLF4和β-连环蛋白的表达呈负相关。本研究表明，胃癌组织中β-连环蛋白表达显著增加，KLF4蛋白表达明显降低，表明胃癌中KLF4的表达与β-连环蛋白的表达呈负相关。这两种蛋白的表达改变与胃癌的肿瘤晚期有关。
