Hall T J, Chambers T J
Merrell Dow Research Institute, Strasbourg, France.
Bone Miner. 1990 Mar;8(3):211-6. doi: 10.1016/0169-6009(90)90106-p.
Gallium nitrate has been used clinically to treat cancer-related hypercalcemia. It has been suggested that gallium may reduce calcium release from bone by inhibiting bone resorption, but the mechanism(s) involved remain to be elucidated. Therefore, we have examined the effect of gallium on bone resorption in vitro using osteoclasts isolated from neonatal rat long bones cultured on slices of cortical bone. Gallium nitrate (0.01-100 micrograms/ml) produced a concentration-dependent inhibition of bone resorption. Morphological studies showed that even (100 micrograms/ml) gallium nitrate induced no light microscopical change in osteoclast morphology and did not affect their survival on bone slices. Pretreatment of bone slices with gallium nitrate (100 micrograms/ml for 18 h), followed by extensive washing also inhibited subsequent osteoclastic bone resorption. These results suggest that gallium can be adsorbed onto the calcified surface of bone and inhibit osteoclastic bone resorption.
硝酸镓已被临床用于治疗癌症相关的高钙血症。有人提出，镓可能通过抑制骨吸收来减少骨中钙的释放，但其涉及的机制仍有待阐明。因此，我们使用从新生大鼠长骨分离并培养在皮质骨切片上的破骨细胞，在体外研究了镓对骨吸收的影响。硝酸镓（0.01 - 100微克/毫升）对骨吸收产生浓度依赖性抑制作用。形态学研究表明，即使是（100微克/毫升）硝酸镓也未引起破骨细胞形态的光学显微镜变化，并且不影响它们在骨切片上的存活。用硝酸镓（100微克/毫升，处理18小时）预处理骨切片，然后大量冲洗，也抑制了随后的破骨细胞性骨吸收。这些结果表明，镓可以吸附到骨的钙化表面并抑制破骨细胞性骨吸收。
