Soto Esteban, Illanes Oscar, Hilchie David, Morales Juan A, Sunyakumthorn Piyanate, Hawke John P, Goodwin Andrew E, Riggs Allen, Yanong Roy P, Pouder Deborah B, Francis-Floyd Ruth, Arauz Maziel, Bogdanovic Lewis, Castillo-Alcala Fernanda
Ross University, School of Veterinary Medicine, Department of Pathobiology, PO Box 334, Basseterre, St. Kitts, West Indies.
J Vet Diagn Invest. 2012 Sep;24(5):840-5. doi: 10.1177/1040638712452108. Epub 2012 Jul 11.
Members of the genus Francisella (viz., F. noatunensis subsp. orientalis [Fno] and F. noatunensis subsp. noatunensis) have been described as causative agents of chronic granulomatous and pyogranulomatous lesions in wild and cultured fish species. In the present study, 68 archived formalin-fixed, paraffin-embedded (FFPE) tissues from several fish species, collected at different geographical locations from 2000 to 2011, were analyzed using a real-time polymerase chain reaction assay for the detection of the Fno intracellular growth loci C (iglC) gene and by immunohistochemistry for the demonstration of Fno antigens. The results revealed a high correlation between these 2 diagnostic techniques validating their use for the diagnosis of Fno infection in archived FFPE tissues and confirming the presence of Fno in fish species from the Cari y years of the present century.
弗朗西斯菌属的成员（即东方诺氏弗朗西斯菌亚种[Fno]和诺氏弗朗西斯菌诺氏亚种）已被描述为野生和养殖鱼类慢性肉芽肿性和脓性肉芽肿性病变的病原体。在本研究中，对2000年至2011年从不同地理位置采集的几种鱼类的68份存档福尔马林固定石蜡包埋（FFPE）组织进行了分析，采用实时聚合酶链反应检测法检测Fno细胞内生长位点C（iglC）基因，并通过免疫组织化学法检测Fno抗原。结果显示这两种诊断技术之间具有高度相关性，验证了它们在存档FFPE组织中诊断Fno感染的用途，并证实了本世纪最初几年鱼类中存在Fno。
