Gonçalves Lucas Amorim, de Castro Soares Siomar, Pereira Felipe Luiz, Dorella Fernanda Alves, de Carvalho Alex Fiorini, de Freitas Almeida Gabriel Magno, Leal Carlos Augusto Gomes, Azevedo Vasco, Figueiredo Henrique César Pereira
National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil ; Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais, Belo Horizonte, Brazil.
National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Stand Genomic Sci. 2016 Apr 12;11:30. doi: 10.1186/s40793-016-0151-0. eCollection 2016.
The genus Francisella is composed of Gram-negative, pleomorphic, strictly aerobic and non-motile bacteria, which are capable of infecting a variety of terrestrial and aquatic animals, among which Francisella noatunensis subsp. orientalis stands out as the causative agent of pyogranulomatous and granulomatous infections in fish. Accordingly, F. noatunensis subsp. orientalis is responsible for high mortality rates in freshwater fish, especially Nile Tilapia. In the current study, we present the genome sequences of F. noatunensis subsp. orientalis strains FNO12, FNO24 and FNO190. The genomes include one circular chromosome of 1,859,720 bp, consisting of 32 % GC content, 1538 coded proteins and 363 pseudogenes for FNO12; one circular chromosome of 1,862,322 bp, consisting of 32 % GC content, 1537 coded proteins and 365 pseudogenes for FNO24; and one circular chromosome of 1,859,595 bp, consisting of 32 % GC content, 1539 coded proteins and 362 pseudogenes for FNO190. All genomes have similar genetic content, implicating a clonal-like behavior for this species.
弗朗西斯菌属由革兰氏阴性、多形性、严格需氧且不运动的细菌组成，这些细菌能够感染多种陆生和水生动物，其中东方诺氏弗朗西斯菌作为鱼类脓性肉芽肿和肉芽肿感染的病原体而备受关注。因此，东方诺氏弗朗西斯菌是淡水鱼，尤其是尼罗罗非鱼高死亡率的原因。在本研究中，我们展示了东方诺氏弗朗西斯菌菌株FNO12、FNO24和FNO190的基因组序列。对于FNO12，基因组包括一条1,859,720 bp的环状染色体，GC含量为32%，有1538个编码蛋白和363个假基因；对于FNO24，基因组包括一条1,862,322 bp的环状染色体，GC含量为32%，有1537个编码蛋白和365个假基因；对于FNO190，基因组包括一条1,859,595 bp的环状染色体，GC含量为32%，有1539个编码蛋白和362个假基因。所有基因组具有相似的遗传内容，表明该物种具有类似克隆的行为。
