Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Int J Dermatol. 2012 Aug;51(8):923-9. doi: 10.1111/j.1365-4632.2011.05214.x.
Transmembrane delivery of active peptides and proteins, including skin delivery of cosmeceutical proteins such as collagen and elastin, has been a challenging issue. Amphipathic cell-penetrating peptides (CPPs) have been proposed as carrier peptides to mediate cellular uptake of proteins without covalent binding.
In this study, we have used a short peptide, Pep-1, as our CPP to transport elastin into fibroblast cells. Different ratios of Pep-1/elastin complexes were produced by using a fixed amount of elastin and different molar ratio of Pep-1. The ability of transduction into cells was determined by fluorescence microscopy. The characteristics of Pep-1/elastin complexes were monitored using scanning electron microscopy and photon correlation spectroscopy.
No cellular toxicity was observed in cells treated with Pep-1/elastin complex. Finally, we determined a Pep-1 : elastin ratio of 10 : 1 as the most effective ratio in cellular delivery of elastin.
Pep-1 mediated fast and effective delivery of elastin as a cosmetic protein into fibroblast cells in the treatment of skin-aging symptoms.
活性肽和蛋白质的跨膜递送，包括胶原蛋白和弹性蛋白等美容蛋白的皮肤递送，一直是一个具有挑战性的问题。两亲性细胞穿透肽（CPPs）已被提议作为载体肽，以介导蛋白质的细胞摄取而不发生共价结合。
在这项研究中，我们使用短肽 Pep-1 作为 CPP 将弹性蛋白输送到成纤维细胞中。通过使用固定量的弹性蛋白和不同摩尔比的 Pep-1 来制备不同比例的 Pep-1/弹性蛋白复合物。通过荧光显微镜测定转导进入细胞的能力。使用扫描电子显微镜和光子相关光谱法监测 Pep-1/弹性蛋白复合物的特性。
用 Pep-1/弹性蛋白复合物处理的细胞没有观察到细胞毒性。最后，我们确定 Pep-1:弹性蛋白的比例为 10:1 是弹性蛋白细胞递送中最有效的比例。
Pep-1 介导的弹性蛋白作为美容蛋白快速有效地递送到成纤维细胞中，用于治疗皮肤衰老症状。
