Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran.
Chem Biol Drug Des. 2013 Aug;82(2):189-95. doi: 10.1111/cbdd.12150.
Transdermal drug delivery of proteins is challenging because the skin acts as a natural and protective barrier. Several techniques including using the cell-penetrating peptides have been studied to increase the penetration of therapeutic proteins into and through the skin. Cell-penetrating peptides facilitate and improve the transduction of large and hydrophilic cargo molecules through plasma membrane. We have recently reported an efficient skin delivery of elastin protein in complex with a cell-penetrating peptide called Pep-1. As the biophysical characteristics of cell-penetrating peptide/protein complexes have been linked with their biological responses, in this study, we investigated biophysical properties of Pep-1/elastin complexes (ratio 10:1) stored in three temperatures (-20 °C, 4 °C and 25 °C) by photon correlation spectroscopy, circular dichroism and isothermal denaturation. We also evaluated the ability of transduction of this complex into cells and skin tissue using both fluorescence microscopy and Kodak In-Vivo FX Pro Imaging System.
蛋白质经皮给药具有挑战性，因为皮肤是天然的保护屏障。已经研究了几种技术，包括使用穿透肽来增加治疗性蛋白质穿透和穿过皮肤的能力。穿透肽有助于并改善大亲水性货物分子穿过质膜的转导。我们最近报道了一种与称为 Pep-1 的穿透肽复合的弹性蛋白的有效皮肤给药。由于穿透肽/蛋白质复合物的生物物理特性与其生物学反应相关，因此在这项研究中，我们通过光子相关光谱法、圆二色性和等温变性研究了 Pep-1/弹性蛋白复合物（比例为 10:1）在三种温度（-20°C、4°C 和 25°C）下的储存的生物物理特性。我们还使用荧光显微镜和柯达 In-Vivo FX Pro 成像系统评估了该复合物转导进入细胞和皮肤组织的能力。
