Institute of Veterinary Anatomy, University of Zürich, Switzerland.
Virology. 2012 Oct 25;432(2):353-60. doi: 10.1016/j.virol.2012.06.020. Epub 2012 Jul 11.
Herpes simplex virus type 1 capsids bud at nuclear and Golgi membranes for envelopment by phospholipid bilayers. In the absence of U(S)3, nuclear membranes form multiple folds harboring virions that suggests disturbance in membrane turnover. Therefore, we investigated phospholipid metabolism in cells infected with the U(S)3 deletion mutant R7041(ΔU(S)3), and quantified membranes involved in viral envelopment. We report that (i) [(3)H]-choline incorporation into nuclear membranes and cytoplasmic membranes was enhanced peaking at 12 or 20 h post inoculation with wild type HSV-1 and R7041(ΔU(S)3), respectively, (ii) the surface area of nuclear membranes increased until 24 h of R7041(ΔU(S)3) infection forming folds that equaled ~45% of the nuclear surface, (iii) the surface area of viral envelopes between nuclear membranes equaled ~2400 R7041(ΔU(S)3) virions per cell, and (iv) during R7041(ΔU(S)3) infection, the Golgi complex expanded dramatically. The data indicate that U(S)3 plays a significant role in regulation of membrane biosynthesis.
单纯疱疹病毒 1 型衣壳在核膜和高尔基体膜上出芽，以便被磷脂双层膜包裹。在没有 U(S)3 的情况下，核膜会形成多个含有病毒的褶皱，这表明膜周转率受到干扰。因此，我们研究了感染 U(S)3 缺失突变体 R7041(ΔU(S)3)的细胞中的磷脂代谢，并定量了参与病毒包膜形成的膜。我们报告说：(i) 在用野生型 HSV-1 和 R7041(ΔU(S)3)接种后 12 或 20 小时，[(3)H]-胆碱分别掺入核膜和质膜的含量增加，(ii) 核膜的表面积增加，直到 R7041(ΔU(S)3)感染 24 小时，形成约占核表面积 45%的褶皱，(iii) 核膜之间病毒包膜的表面积相当于每个细胞约 2400 个 R7041(ΔU(S)3)病毒粒子，以及 (iv) 在 R7041(ΔU(S)3)感染期间，高尔基体复合物显著扩张。这些数据表明 U(S)3 在调节膜生物合成中发挥了重要作用。
