产黄青霉中δ-(α-氨基己二酰基)半胱氨酰缬氨酸中氨基酸的绝对构型。

The absolute configuration of the amino acids in delta-(alpha-aminoadipyl)cysteinylvaline from Penicillium chrysogenum.

作者信息

Chan J A, Huang F C, Sih C J

出版信息

Biochemistry. 1976 Jan 13;15(1):177-80. doi: 10.1021/bi00646a027.

Abstract

Radioactive carbon-14 L-alpha-aminoadipic acid, L-cysteine, or L-valine were readily incorporated into the intracellular tripeptide, delta-(alpha-aminoadipyl)cysteinylvaline (ACV), by washed starved cells of Penicillium chrysogenum. The labeled ACV in each case was oxidized with performic acid and isolated as its corresponding sulfonic acid derivative. After acid hydrolysis, the configuration of the component acids was determined by L- and D-amino acid oxidases, which showed the tripeptide (ACV) from P. chrysogenum to be delta-(L-aminoadipyl)-L-cysteinyl-D-valine.

摘要

放射性碳-14标记的L-α-氨基己二酸、L-半胱氨酸或L-缬氨酸很容易被产黄青霉的饥饿洗涤细胞掺入细胞内三肽δ-(α-氨基己二酰基)半胱氨酰缬氨酸（ACV）中。在每种情况下，标记的ACV用过甲酸氧化，并分离为其相应的磺酸衍生物。酸水解后，通过L-和D-氨基酸氧化酶确定组成酸的构型，结果表明产黄青霉的三肽（ACV）为δ-(L-氨基己二酰基)-L-半胱氨酰-D-缬氨酸。