产黄青霉发酵液中δ-（L-α-氨基己二酰基）-L-半胱氨酰-D-缬氨酸的存在情况
Presence of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine in fermentations of Penicillium chrysogenum.
作者信息
Adriaens P, Meesschaert B, Wuyts W, Vanderhaeghe H, Eyssen H
出版信息
Antimicrob Agents Chemother. 1975 Dec;8(6):638-42. doi: 10.1128/AAC.8.6.638.
Abstract
Cultures of Penicillium chrysogenum, growth with [(35)S]sulfate or labeled amino acids, were examined by ion-exchange chromatography for possible peptidic precursors of penicillin. A sulfur-containing compound, present in both the mycelial extracts and the culture filtrates, was eluted at the location of the synthetic lld-tripeptide delta-(l-alpha-aminoadipyl)-l-cysteinyl-d-valine. Since this compound was also labeled when the cultures were incubated with dl-[6-(14)C]alpha-aminoadipic acid, l-[3,3'-(3)H]cystine, or dl-[1-(14)C]valine, its identity with the synthetic lld-tripeptide can be accepted. No delta-(l-alpha-aminoadipyl)-l-cysteine or lll-tripeptide were detected. The implications of these findings for tripeptide and penicillin biosynthesis are discussed.
摘要
用离子交换色谱法对产黄青霉培养物（用[35S]硫酸盐或标记氨基酸培养）进行检测，以寻找青霉素可能的肽类前体。在菌丝提取物和培养滤液中均存在一种含硫化合物，其洗脱位置与合成的lld-三肽δ-（l-α-氨基己二酰基）-l-半胱氨酰基-d-缬氨酸相同。由于当培养物与dl-[6-(14)C]α-氨基己二酸、l-[3,3'-(3)H]胱氨酸或dl-[1-(14)C]缬氨酸一起孵育时该化合物也被标记，因此可以认为它与合成的lld-三肽是同一物质。未检测到δ-（l-α-氨基己二酰基）-l-半胱氨酸或lll-三肽。讨论了这些发现对三肽和青霉素生物合成的意义。
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The isolation of D-valine from Penicillium chrysogenum.
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The biosynthesis of penicillin. 7. Further experiments on the utilization of L-and D-valine and the effect of cystine and valine analogues on penicillin synthesis.青霉素的生物合成。7. 关于L-和D-缬氨酸利用以及胱氨酸和缬氨酸类似物对青霉素合成影响的进一步实验。
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The biosynthesis of penicillin. 4. The synthesis of benzylpenicillin by washed mycelium of Penicillium chrysogenum.青霉素的生物合成。4. 产黄青霉洗涤菌丝体合成苄青霉素。
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