The Blood Bank, Landspitali University Hospital, Snorrabraut 60, 105 Reykjavik, Iceland.
Int J Biol Macromol. 2012 Nov;51(4):675-80. doi: 10.1016/j.ijbiomac.2012.07.005. Epub 2012 Jul 10.
Chitooligosaccharides are of interest as potential drugs due to their bioactivity and water solubility. We compared the effect of acetylated and deacetylated chitooligomers (Hexamers) on short-term expansion (7 days) and osteogenic differentiation of bone-marrow derived, human mesenchymal stem cells in terms of gene expression, cytokine secretion and quality of osteogenic differentiation. We show that chitooligomers affect hMSC gene expression and cytokine secretion, but not mineralization. The effect of chitooligomers was shown to be dependent on the acetylation degree, with significantly stronger effects when cells are stimulated with chitin-derived Hexamers (N-Acetyl Chitohexaose) than with Chitosan Hexamers (Chitohexaose).
壳寡糖由于其生物活性和水溶性而成为潜在的药物。我们比较了乙酰化和脱乙酰壳寡聚物（六聚体）对骨髓来源的人间充质干细胞短期扩增（7 天）和成骨分化的影响，从基因表达、细胞因子分泌和成骨分化质量方面进行了评估。我们表明壳寡糖会影响 hMSC 的基因表达和细胞因子分泌，但不会影响矿化。壳寡糖的作用被证明依赖于乙酰化程度，当用来源于几丁质的六聚体（N-乙酰壳六糖）刺激细胞时，效果明显强于壳聚糖六聚体（壳六糖）。
