Li Kecheng, Xing Ronge, Liu Song, Qin Yukun, Li Pengcheng
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Nantong Marine Science and Technology R&D Center, IOCAS, Jiangsu 226006, China.
Biomed Res Int. 2017;2017:2486515. doi: 10.1155/2017/2486515. Epub 2017 Jun 5.
Chitohexaose has attracted wide interest due to its special bioactivities and these potential activities are significantly related to N-acetylation. Herein, six chitohexaose fractions with different degrees of acetylation were prepared by selective N-acetylation and ion-exchange chromatography and further analyzed by ESI/MS. It is revealed that all the six N-acetylated chitohexaoses were of single molecular weight, the molecular weights of which were exactly assigned to 1026.44 Da, 1068.44 Da, 1110.48 Da, 1152.48 Da, 1194.49 Da, and 1236.48 Da, respectively. These results suggested that the six prepared N-acetylated chitohexaoses were N-acetylchitohexaose (D5A1), di--acetylchitohexaose (D4A2), tri--acetylchitohexaose (D3A3), tetra--acetylchitohexaose (D2A4), penta--acetylchitohexaose (D1A5), and hexa--acetylchitohexaose (A6), respectively, which are of great significance to screen their bioactivities and discover well-defined chitooligosaccharide molecules as potential drugs.
壳六糖因其特殊的生物活性而备受关注，这些潜在活性与N-乙酰化密切相关。在此，通过选择性N-乙酰化和离子交换色谱法制备了六种不同乙酰化程度的壳六糖馏分，并通过电喷雾电离质谱（ESI/MS）进行了进一步分析。结果表明，所有六种N-乙酰化壳六糖均为单分子量，其分子量分别精确确定为1026.44 Da、1068.44 Da、1110.48 Da、1152.48 Da、1194.49 Da和1236.48 Da。这些结果表明，所制备的六种N-乙酰化壳六糖分别为N-乙酰壳六糖（D5A1）、二乙酰壳六糖（D4A2）、三乙酰壳六糖（D3A3）、四乙酰壳六糖（D2A4）、五乙酰壳六糖（D1A5）和六乙酰壳六糖（A6），这对于筛选它们的生物活性以及发现明确的壳寡糖分子作为潜在药物具有重要意义。
