Department of Oral and Maxillofacial Surgery, Hospital de Cabueñes, Gijón, Asturias, Spain.
Virchows Arch. 2012 Aug;461(2):163-8. doi: 10.1007/s00428-012-1283-2. Epub 2012 Jul 12.
Focal adhesion kinase (FAK) and p53 have been associated with metastatic activity and a poor prognosis in oral squamous cell carcinoma (OSCC). Recently, a feedback mechanism in which FAK regulates p53 has been proposed. The present study aims to determine the role of p53 in FAK regulation in these tumors. FAK and p53 expression was examined by immunohistochemistry in normal oral mucosa and in 67 oral squamous cell carcinomas. p16(INK4a) was also studied in view of its association with human papillomavirus infection. The association between FAK and p53 was subsequently analyzed. FAK expression in OSCCs was heterogeneous: 22 (33 %) cases showed weak expression, 16 (24 %) showed moderate expression, and 22 (33 %) cases showed high expression. Regarding p53, 31 of 67 (46 %) available tumor specimens showed negative staining, and 36 of 67 (54 %) showed positive nuclear staining for p53. FAK expression was inversely correlated with p53 expression (Fisher's exact test, p = 0.005). There was no association between p16(INK4a) and p53 or FAK expression. In conclusion, our results support the hypothesis that FAK activity might be involved in the down-regulation of p53 expression in OSCC.
黏着斑激酶（FAK）和 p53 与口腔鳞状细胞癌（OSCC）的转移活性和预后不良有关。最近，提出了一种反馈机制，即 FAK 调节 p53。本研究旨在确定 p53 在这些肿瘤中 FAK 调节中的作用。通过免疫组织化学法检测正常口腔黏膜和 67 例口腔鳞状细胞癌中 FAK 和 p53 的表达。还研究了 p16（INK4a），因为它与人类乳头瘤病毒感染有关。随后分析了 FAK 和 p53 之间的关联。OSCC 中的 FAK 表达呈异质性：22 例（33%）表达弱，16 例（24%）表达中度，22 例（33%）表达强。关于 p53，67 个可利用肿瘤标本中有 31 个（46%）显示阴性染色，67 个中有 36 个（54%）显示核 p53 阳性染色。FAK 表达与 p53 表达呈负相关（Fisher 确切检验，p=0.005）。p16（INK4a）与 p53 或 FAK 表达之间没有关联。总之，我们的结果支持 FAK 活性可能参与 OSCC 中 p53 表达下调的假说。
