Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia.
Anal Chim Acta. 2012 Aug 13;738:1-12. doi: 10.1016/j.aca.2012.05.052. Epub 2012 Jun 4.
In recent years the use of monolithic polymers in separation science has greatly increased due to the advantages these materials present over particle-based stationary phases, such as their relative ease of preparation and good permeability. For these reasons, these materials present high potential as stationary phases for the separation and purification of large molecules such as proteins, peptides, nucleic acids and cells. An example of this is the wide range of commercial available polymer-based monolithic columns now present in the market. This review summarizes recent developments in the synthesis of monolithic polymers for separation science, such as the incorporation of nanostructures in the polymeric scaffold as well as the preparation of hybrid structures. The different methods used in the surface functionalization of monolithic columns are also reviewed. Finally, we critically discuss the recent applications of this column technology in the separation of large molecules under different chromatographic mode.
近年来，由于整体聚合物相对于基于颗粒的固定相具有诸多优势，如相对容易制备和良好的渗透性，因此在分离科学中越来越多地使用整体聚合物。基于这些原因，这些材料具有作为分离和纯化大分子（如蛋白质、肽、核酸和细胞）的固定相的巨大潜力。例如，市场上现在有广泛的商业可用的聚合物整体柱。本文综述了近年来用于分离科学的整体聚合物的合成进展，如在聚合物支架中引入纳米结构以及制备杂化结构。还综述了整体柱表面功能化的不同方法。最后，我们批判性地讨论了该柱技术在不同色谱模式下分离大分子的最新应用。
