Liu Ying-hua, Zhang Yong, Xu Qing, Yu Xiao-ming, Zhang Xin-sheng, Wang Jin, Xue Chao, Yang Xue-yan, Zhang Rong-xin, Xue Chang-yong
Department of Nutrition, Chinese PLA General Hospital, and Peking University Health Science, Beijing, China.
Biosci Biotechnol Biochem. 2012;76(6):1213-8. doi: 10.1271/bbb.120079. Epub 2012 Jun 7.
A further investigation of the lipolysis induced by medium-chain triglyceride (MCT) was conducted on C57BL/6J mice fed with a diet containing 2% MCT or 2% long-chain triglyceride (LCT). Blood norepinephrine, body fat and blood lipid variables, and the protein or mRNA expression of the genes relevant to lipolysis were measured and analyzed in the white and brown adipose tissue (WAT, BAT). Decreased body fat and improved blood lipid profiles attributable to MCT were confirmed. A higher level of blood norepinephrine was observed with the MCT diet. The adipose triglyceride lipase (ATGL) activity and its mRNA expression, the expression of protein and mRNA of the beta 3 adrenergic receptor (β3-AR) in both WAT and BAT, and the hormone-sensitive lipase (HSL) activity and its mRNA expression in BAT were significantly increased in the mice with MCT feeding. The lipolysis induced by MCT might be partially mediated by increasing norepinephrine, thereafter signaling the up-regulation of β3-AR, ATGL, and HSL in WAT and BAT.
对喂食含2%中链甘油三酯（MCT）或2%长链甘油三酯（LCT）饮食的C57BL/6J小鼠进行了关于中链甘油三酯诱导脂解作用的进一步研究。测量并分析了白色和棕色脂肪组织（WAT、BAT）中的血去甲肾上腺素、体脂和血脂变量，以及与脂解相关基因的蛋白质或mRNA表达。证实了MCT可降低体脂并改善血脂状况。观察到MCT饮食组血去甲肾上腺素水平较高。MCT喂养的小鼠中，白色和棕色脂肪组织中的脂肪甘油三酯脂肪酶（ATGL）活性及其mRNA表达、β3肾上腺素能受体（β3-AR）的蛋白质和mRNA表达，以及棕色脂肪组织中的激素敏感性脂肪酶（HSL）活性及其mRNA表达均显著增加。MCT诱导的脂解作用可能部分是通过增加去甲肾上腺素介导的，进而促使白色和棕色脂肪组织中的β3-AR、ATGL和HSL上调。
