Shu-Fen Han, Jun Jiao, Wei Zhang, Jia-Ying Xu, Weiguo Zhang, Chun-Ling Fu, Li-Qiang Qin
Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China; Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Disease, School of Public Health, Soochow University, Suzhou, China.
Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China.
Nutrition. 2017 Jan;33:118-124. doi: 10.1016/j.nut.2016.05.006. Epub 2016 Jun 2.
Dietary fiber consumption is associated with reduced risk for the development of noncommunicable diseases. The aim of the present study was to evaluate the effects of cereal dietary fiber on the levels of proteins involved in lipolysis and thermogenesis in white adipose tissue (WAT) and brown adipose tissue (BAT) of C57 BL/6 J mice fed a high-fat diet (HFD).
Male C57BL/6 J mice were fed normal chow diet (Chow), HFD, HFD plus oat fiber (H-oat), or HFD plus wheat bran fiber (H-wheat) for 24 wk. Body weight and food intake were recorded weekly. Serum adiponectin was assayed by an enzyme-linked immunosorbent assay kit. Western blotting was used to assess the protein expressions of adipose triacylglycerol lipase (ATGL), cAMP protein kinase catalytic subunit (cAMP), protein kinase A (PKA), perilipin A, hormone-sensitive lipase (HSL), uncoupling protein 1 (UCP1), fibroblast growth factor 21 (FGF-21), β3-adrenergic receptor (β3AR), and proliferator-activated receptor gamma coactivator-1 α (PGC-1 α) in the WAT and BAT.
At the end of the feeding period, body and adipose tissues weight in both H-oat and H-wheat groups were lower than in the HFD group. Mice in the H-oat and H-wheat groups showed an increasing trend in serum adiponectin level. Compared with the HFD group, cereal dietary fiber increased protein expressions involved in the lipolysis and browning process. Compared with the H-wheat group, H-oat was more effective in protein expressions of PKA, PGC-1 α, and UCP1 of the WAT samples. Compared with the H-oat group, H-wheat was more effective in protein expressions of PKA, ATGL, UCP1, β3AR, and FGF-21 of the BAT samples.
Taken together, our results suggested that cereal dietary fiber enhanced adipocyte lipolysis by the cAMP-PKA-HSL pathway and promoted WAT browning by activation of UCP1, and consequently reduced visceral fat mass in response to HFD feeding.
膳食纤维的摄入与降低非传染性疾病的发生风险相关。本研究旨在评估谷物膳食纤维对高脂饮食（HFD）喂养的C57 BL/6 J小鼠白色脂肪组织（WAT）和棕色脂肪组织（BAT）中参与脂肪分解和产热的蛋白质水平的影响。
雄性C57BL/6 J小鼠分别喂食正常饲料（Chow）、HFD、HFD加燕麦纤维（H-oat）或HFD加麦麸纤维（H-wheat），持续24周。每周记录体重和食物摄入量。采用酶联免疫吸附测定试剂盒检测血清脂联素。蛋白质印迹法用于评估WAT和BAT中脂肪甘油三酯脂肪酶（ATGL）、cAMP蛋白激酶催化亚基（cAMP）、蛋白激酶A（PKA）、围脂滴蛋白A、激素敏感性脂肪酶（HSL）、解偶联蛋白1（UCP1）、成纤维细胞生长因子21（FGF-21）、β3-肾上腺素能受体（β3AR）和增殖激活受体γ辅激活因子-1α（PGC-1α）的蛋白表达。
喂养期结束时，H-oat组和H-wheat组的体重和脂肪组织重量均低于HFD组。H-oat组和H-wheat组小鼠的血清脂联素水平呈上升趋势。与HFD组相比，谷物膳食纤维增加了参与脂肪分解和褐变过程的蛋白表达。与H-wheat组相比，H-oat对WAT样本中PKA、PGC-1α和UCP1的蛋白表达更有效。与H-oat组相比，H-wheat对BAT样本中PKA、ATGL、UCP1、β3AR和FGF-21的蛋白表达更有效。
综上所述，我们的结果表明，谷物膳食纤维通过cAMP-PKA-HSL途径增强脂肪细胞的脂肪分解，并通过激活UCP1促进WAT褐变，从而降低HFD喂养引起的内脏脂肪量。
