Saitou Hiroki, Watanabe Manami, Maruyama Kiyofumi
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan.
Biosci Biotechnol Biochem. 2012;76(6):1239-41. doi: 10.1271/bbb.120054. Epub 2012 Jun 7.
Gene phlG encoding 2,4-diacetylphloroglucinol hydrolase was cloned from Pseudomonas sp. YGJ3 and expressed in Escherichia coli. Recombinant PhlG was purified homogeneously. It required 2-mercaptoethanol for stability. Km for 2,4-diacetylphloroglucinol and kcat were determined to be 24 µM and 5.8 s(-1) respectively. CoCl2 specifically and significantly activated PhlG.
从假单胞菌属菌株YGJ3中克隆出编码2,4-二乙酰间苯三酚水解酶的基因phlG，并在大肠杆菌中表达。重组PhlG被纯化为均一形式。它需要2-巯基乙醇来维持稳定性。2,4-二乙酰间苯三酚的米氏常数（Km）和催化常数（kcat）分别测定为24 μM和5.8 s(-1)。氯化钴能特异性且显著地激活PhlG。
