来自假单胞菌属YGJ3的2,4-二乙酰基间苯三酚水解酶（PhlG）的分子和催化特性
Molecular and catalytic properties of 2,4-diacetylphloroglucinol hydrolase (PhlG) from Pseudomonas sp. YGJ3.
作者信息
Saitou Hiroki, Watanabe Manami, Maruyama Kiyofumi
机构信息
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan.
出版信息
Biosci Biotechnol Biochem. 2012;76(6):1239-41. doi: 10.1271/bbb.120054. Epub 2012 Jun 7.
PMID:22790955
Abstract
Gene phlG encoding 2,4-diacetylphloroglucinol hydrolase was cloned from Pseudomonas sp. YGJ3 and expressed in Escherichia coli. Recombinant PhlG was purified homogeneously. It required 2-mercaptoethanol for stability. Km for 2,4-diacetylphloroglucinol and kcat were determined to be 24 µM and 5.8 s(-1) respectively. CoCl2 specifically and significantly activated PhlG.
摘要
从假单胞菌属菌株YGJ3中克隆出编码2,4-二乙酰间苯三酚水解酶的基因phlG，并在大肠杆菌中表达。重组PhlG被纯化为均一形式。它需要2-巯基乙醇来维持稳定性。2,4-二乙酰间苯三酚的米氏常数（Km）和催化常数（kcat）分别测定为24 μM和5.8 s(-1)。氯化钴能特异性且显著地激活PhlG。
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