• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Orosphere assay: a method for propagation of head and neck cancer stem cells. Orosphere assay：一种用于头颈部癌症干细胞增殖的方法。
Head Neck. 2013 Jul;35(7):1015-21. doi: 10.1002/hed.23076. Epub 2012 Jul 13.
2
Endothelial cell-initiated signaling promotes the survival and self-renewal of cancer stem cells.内皮细胞起始的信号转导促进了癌症干细胞的存活和自我更新。
Cancer Res. 2010 Dec 1;70(23):9969-78. doi: 10.1158/0008-5472.CAN-10-1712. Epub 2010 Nov 23.
3
The biology of head and neck cancer stem cells.头颈部癌症干细胞的生物学特性。
Oral Oncol. 2012 Jan;48(1):1-9. doi: 10.1016/j.oraloncology.2011.10.004. Epub 2011 Nov 8.
4
Cancer stem cells enrichment with surface markers CD271 and CD44 in human head and neck squamous cell carcinomas.人类头颈部鳞状细胞癌中表面标志物 CD271 和 CD44 的癌症干细胞富集。
Carcinogenesis. 2020 Jun 17;41(4):458-466. doi: 10.1093/carcin/bgz182.
5
Cancer stem cell mediated acquired chemoresistance in head and neck cancer can be abrogated by aldehyde dehydrogenase 1 A1 inhibition.醛脱氢酶1 A1抑制可消除头颈部癌中癌症干细胞介导的获得性化疗耐药性。
Mol Carcinog. 2017 Feb;56(2):694-711. doi: 10.1002/mc.22526. Epub 2016 Aug 4.
6
Cisplatin induces Bmi-1 and enhances the stem cell fraction in head and neck cancer.顺铂可诱导头颈部癌中的Bmi-1表达并增加其干细胞比例。
Neoplasia. 2014 Feb;16(2):137-46. doi: 10.1593/neo.131744.
7
Identification, expansion and characterization of cancer cells with stem cell properties from head and neck squamous cell carcinomas.对头颈部鳞状细胞癌中具有干细胞特性的癌细胞进行鉴定、扩增及特性分析。
Exp Cell Res. 2016 Oct 15;348(1):75-86. doi: 10.1016/j.yexcr.2016.09.003. Epub 2016 Sep 9.
8
CD44(high) /ALDH1(high) head and neck squamous cell carcinoma cells exhibit mesenchymal characteristics and GSK3β-dependent cancer stem cell properties.CD44（高表达）/ALDH1（高表达）的头颈部鳞状细胞癌细胞表现出间充质特征和GSK3β依赖性癌症干细胞特性。
J Oral Pathol Med. 2016 Mar;45(3):180-8. doi: 10.1111/jop.12348. Epub 2015 Sep 24.
9
Cancer stem cells: mediators of tumorigenesis and metastasis in head and neck squamous cell carcinoma.癌症干细胞：头颈部鳞状细胞癌肿瘤发生和转移的介质
Head Neck. 2015 Mar;37(3):317-26. doi: 10.1002/hed.23600. Epub 2014 Mar 25.
10
Head and neck cancer stem cells.头颈部癌症干细胞。
J Dent Res. 2012 Apr;91(4):334-40. doi: 10.1177/0022034511423393. Epub 2011 Sep 20.

引用本文的文献

1
Comparative evaluation of anticancer potential of Spilanthes paniculata leaf and flower essential oil using annexin V and orosphere formation in oral cancer cell line.采用 Annexin V 和球体形成法比较评估麻疯树叶和花精油的抗癌潜力在口腔癌细胞系中。
Med Oncol. 2024 Oct 5;41(11):259. doi: 10.1007/s12032-024-02523-1.
2
Cancer Stem Cells in Oral Squamous Cell Carcinoma: A Narrative Review on Experimental Characteristics and Methodological Challenges.口腔鳞状细胞癌中的癌症干细胞：关于实验特征和方法学挑战的叙述性综述
Biomedicines. 2024 Sep 16;12(9):2111. doi: 10.3390/biomedicines12092111.
3
A Kinome-Wide Synthetic Lethal CRISPR/Cas9 Screen Reveals That mTOR Inhibition Prevents Adaptive Resistance to CDK4/CDK6 Blockade in HNSCC.全基因组激酶的合成致死 CRISPR/Cas9 筛选揭示，mTOR 抑制可防止头颈部鳞状细胞癌对 CDK4/CDK6 阻断的适应性耐药。
Cancer Res Commun. 2024 Jul 1;4(7):1850-1862. doi: 10.1158/2767-9764.CRC-24-0247.
4
Advances in 3D Culture Models to Study Exosomes in Triple-Negative Breast Cancer.用于研究三阴性乳腺癌中外泌体的3D培养模型的进展
Cancers (Basel). 2024 Feb 22;16(5):883. doi: 10.3390/cancers16050883.
5
Three-dimensional (3D) cell culture: a valuable step in advancing treatments for human hepatocellular carcinoma.三维（3D）细胞培养：推进人类肝细胞癌治疗的重要一步。
Cancer Cell Int. 2022 Jul 30;22(1):243. doi: 10.1186/s12935-022-02662-3.
6
Graphene-Based Materials for Efficient Neurogenesis.基于石墨烯的材料促进神经发生。
Adv Exp Med Biol. 2022;1351:43-64. doi: 10.1007/978-981-16-4923-3_3.
7
The IL-6R and Bmi-1 axis controls self-renewal and chemoresistance of head and neck cancer stem cells.IL-6R 和 Bmi-1 轴控制头颈部癌症干细胞的自我更新和化疗耐药性。
Cell Death Dis. 2021 Oct 23;12(11):988. doi: 10.1038/s41419-021-04268-5.
8
SOD2 Enhancement by Long-Term Inhibition of the PI3K Pathway Confers Multi-Drug Resistance and Enhanced Tumor-Initiating Features in Head and Neck Cancer.长期抑制 PI3K 通路增强 SOD2 表达赋予头颈部癌细胞多药耐药性和增强的肿瘤起始特性。
Int J Mol Sci. 2021 Oct 19;22(20):11260. doi: 10.3390/ijms222011260.
9
Organoids in Tissue Transplantation.类器官在组织移植中的应用。
Adv Exp Med Biol. 2021;1347:45-64. doi: 10.1007/5584_2021_647.
10
Propolis reduces the stemness of head and neck squamous cell carcinoma.蜂胶降低头颈部鳞状细胞癌的干性。
Arch Oral Biol. 2021 May;125:105087. doi: 10.1016/j.archoralbio.2021.105087. Epub 2021 Feb 19.

本文引用的文献

1
Three-dimensional spheroid culture of human gingiva-derived mesenchymal stem cells enhances mitigation of chemotherapy-induced oral mucositis.三维球体培养人牙龈间充质干细胞增强减轻化疗诱导的口腔黏膜炎。
Stem Cells Dev. 2012 Apr 10;21(6):937-47. doi: 10.1089/scd.2011.0252. Epub 2011 Jul 28.
2
Eyes wide open: a critical review of sphere-formation as an assay for stem cells.眼睛睁得大大的：球体形成作为干细胞检测方法的批判性评价。
Cell Stem Cell. 2011 May 6;8(5):486-98. doi: 10.1016/j.stem.2011.04.007.
3
Enhanced isolation of adult thymic epithelial cell subsets for multiparameter flow cytometry and gene expression analysis.增强成人胸腺上皮细胞亚群的分离用于多参数流式细胞术和基因表达分析。
J Immunol Methods. 2011 Mar 31;367(1-2):85-94. doi: 10.1016/j.jim.2011.02.008. Epub 2011 Feb 24.
4
Endothelial cell-initiated signaling promotes the survival and self-renewal of cancer stem cells.内皮细胞起始的信号转导促进了癌症干细胞的存活和自我更新。
Cancer Res. 2010 Dec 1;70(23):9969-78. doi: 10.1158/0008-5472.CAN-10-1712. Epub 2010 Nov 23.
5
Comprehensive keratin profiling reveals different histopathogenesis of keratocystic odontogenic tumor and orthokeratinized odontogenic cyst.全面的角蛋白分析揭示了角化囊肿型牙源性肿瘤和正角化牙源性囊肿的不同组织发生机制。
Hum Pathol. 2010 Dec;41(12):1718-25. doi: 10.1016/j.humpath.2010.05.007.
6
New advances on critical implications of tumor- and metastasis-initiating cells in cancer progression, treatment resistance and disease recurrence.肿瘤起始细胞和转移起始细胞在癌症进展、治疗耐药和疾病复发中的关键影响的新进展。
Histol Histopathol. 2010 Aug;25(8):1057-73. doi: 10.14670/HH-25.1057.
7
Single-marker identification of head and neck squamous cell carcinoma cancer stem cells with aldehyde dehydrogenase.利用乙醛脱氢酶对头颈部鳞状细胞癌肿瘤干细胞进行单一标志物鉴定。
Head Neck. 2010 Sep;32(9):1195-201. doi: 10.1002/hed.21315.
8
A suspension induction for myocardial differentiation of rat mesenchymal stem cells on various extracellular matrix proteins.悬浮诱导大鼠间充质干细胞向心肌细胞分化在各种细胞外基质蛋白上的研究。
Tissue Eng Part C Methods. 2010 Oct;16(5):979-87. doi: 10.1089/ten.TEC.2009.0218.
9
A subpopulation of CD133(+) cancer stem-like cells characterized in human oral squamous cell carcinoma confer resistance to chemotherapy.在人口腔鳞状细胞癌中，CD133(+) 肿瘤干细胞样细胞亚群具有特征性，赋予其对化疗的耐药性。
Cancer Lett. 2010 Mar 28;289(2):151-60. doi: 10.1016/j.canlet.2009.08.010. Epub 2009 Sep 11.
10
In vitro propagation and characterization of neoplastic stem/progenitor-like cells from human prostate cancer tissue.人前列腺癌组织中肿瘤干细胞/祖细胞样细胞的体外增殖与特性研究
Prostate. 2009 Nov 1;69(15):1683-93. doi: 10.1002/pros.21018.

Orosphere assay：一种用于头颈部癌症干细胞增殖的方法。

Orosphere assay: a method for propagation of head and neck cancer stem cells.

机构信息

Department of Restorative Sciences, University of Michigan School of Dentistry, Ann Arbor, Michigan, USA.

出版信息

Head Neck. 2013 Jul;35(7):1015-21. doi: 10.1002/hed.23076. Epub 2012 Jul 13.

DOI:10.1002/hed.23076
PMID:22791367
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3887391/
Abstract

BACKGROUND

Recent evidence suggests that head and neck squamous cell carcinomas (HNSCCs) harbor a small subpopulation of highly tumorigenic cells, designated cancer stem cells. A limiting factor in cancer stem cell research is the intrinsic difficulty of expanding cells in an undifferentiated state in vitro.

METHODS

Here, we describe the development of the orosphere assay, a method for the study of putative head and neck cancer stem cells. An orosphere is defined as a nonadherent colony of cells sorted from primary HNSCCs or from HNSCC cell lines and cultured in 3-dimensional soft agar or ultralow attachment plates. Aldehyde dehydrogenase activity and CD44 expression were used here as stem cell markers.

RESULTS

This assay allowed for the propagation of head and neck cancer cells that retained stemness and self-renewal.

CONCLUSION

The orosphere assay is well suited for studies designed to understand the pathobiology of head and neck cancer stem cells.

摘要

背景

最近的证据表明，头颈部鳞状细胞癌（HNSCC）中存在一小部分具有高肿瘤形成能力的细胞，被称为癌症干细胞。癌症干细胞研究的一个限制因素是在体外将细胞在未分化状态下扩增的内在困难。

方法

在这里，我们描述了球体形成测定法的开发，这是一种研究头颈部癌症干细胞的方法。球体是指从原发性 HNSCC 或 HNSCC 细胞系中分离出来的非贴壁细胞集落，并在 3 维软琼脂或超低附着平板中培养。在这里，醛脱氢酶活性和 CD44 表达被用作干细胞标记物。

结果

该测定法允许头颈部癌细胞的增殖，这些细胞保留了干性和自我更新能力。

结论

球体形成测定法非常适合用于研究头颈部癌症干细胞的病理生物学。