Sriram Muthu Irulappan, Kalishwaralal Kalimuthu, Gurunathan Sangiliyandi
Division of Molecular and Cellular Biology, Department of Biotechnology, Kalasalingam University (Kalasalingam Academy of Research and Education), Krishnankoil, Tamilnadu, India.
Methods Mol Biol. 2012;906:33-43. doi: 10.1007/978-1-61779-953-2_3.
Owing to the wide-ranging applications of noble metal nanoparticles in diverse areas of science and technology, different methods have been proposed for their synthesis. Here, we describe the methods for the intracellular biosynthesis of silver and gold nanoparticles using the bacterium Bacillus licheniformis KK2 and this same procedure can be followed for other bacteria as well. The biological synthesis of nanoparticles is highly eco-friendly and possesses distinct advantages such as enhanced stability, better control over the size, shape, and monodispersity of the nanoparticles, when compared with the more traditional physical and chemical methods which often involves the use of hazardous chemicals creating environmental concern.
由于贵金属纳米颗粒在科学技术的各个领域有着广泛的应用，人们已经提出了不同的合成方法。在此，我们描述了利用地衣芽孢杆菌KK2进行银和金纳米颗粒细胞内生物合成的方法，同样的程序也可用于其他细菌。与通常涉及使用有害化学物质从而引发环境问题的传统物理和化学方法相比，纳米颗粒的生物合成具有高度的生态友好性，并且具有诸如增强稳定性、更好地控制纳米颗粒的尺寸、形状和单分散性等明显优势。
