Bowman Linda, Castranova Vincent, Ding Min
Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV, USA.
Methods Mol Biol. 2012;906:415-22. doi: 10.1007/978-1-61779-953-2_34.
Comet assay provides a rapid and reliable way to screen genotoxic effects of a wide variety of nanoparticles. Single cell gel electrophoresis assay (SCGE) or comet assay gained in popularity as a standard technique and is widely used for testing novel particles or chemicals for genotoxicity, monitoring environmental contamination with genotoxins, human biomonitoring and molecular epidemiology, and fundamental research in DNA damage and repair. Here, we describe the method of alkaline comet assay to detect nanoparticle-induced DNA damage.
彗星试验提供了一种快速且可靠的方法，用于筛选多种纳米颗粒的遗传毒性效应。单细胞凝胶电泳试验（SCGE）或彗星试验作为一种标准技术而受到欢迎，并广泛用于测试新型颗粒或化学物质的遗传毒性、监测遗传毒素对环境的污染、人体生物监测和分子流行病学，以及DNA损伤与修复的基础研究。在此，我们描述碱性彗星试验检测纳米颗粒诱导DNA损伤的方法。
