纳米遗传毒理学中的单细胞凝胶电泳（彗星）试验
Single-cell gel electrophoresis (Comet) assay in nano-genotoxicology.
作者信息
Pacurari Maricica, Castranova Vincent
机构信息
Mary Babb Cancer Center, School of Medicine, West Virginia University, Morgantown, WV, USA.
出版信息
Methods Mol Biol. 2012;926:57-67. doi: 10.1007/978-1-62703-002-1_4.
PMID:22975956
Abstract
The Comet assay, or single-cell gel electrophoresis assay, is an easy and simple yet reliable method to evaluate DNA damage in cells. Under the alkaline conditions of this method, DNA strand breaks and alkaline-labile sites are detected. Here we describe the alkaline version of the Comet assay with applications in testing nanoparticles.
摘要
彗星试验，即单细胞凝胶电泳试验，是一种评估细胞中DNA损伤的简便而可靠的方法。在该方法的碱性条件下，可检测到DNA链断裂和碱不稳定位点。在此，我们描述彗星试验的碱性版本及其在纳米颗粒测试中的应用。
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