Department of Pathology, Chi-Mei Medical Center, Tainan, Taiwan.
J Clin Pathol. 2012 Sep;65(9):795-801. doi: 10.1136/jclinpath-2012-200893. Epub 2012 Jul 12.
Low expression of cyclin-dependent kinase 2-associated protein (CDK2AP1) is associated with tumour progression in oral and oesophageal carcinomas, but is not well studied in patients with head and neck cancer and nasopharyngeal carcinoma (NPC).
A rabbit anti-human CDK2AP1 polyclonal antibody was prepared. Immunoblotting of CDK2AP1 was examined in three cell lines and immunoexpression was retrospectively assessed in biopsies of 124 consecutive NPC patients without initial distant metastasis and treated with consistent guidelines.
Higher CDK2AP1 expression level was identified in dysplastic oral keratinocytes, compared with two NPC-derived HONE-1 and TW01 cell lines. Low expression of CDK2AP1 (50.8%) was correlated with advanced nodal status (p=0.002) and American Joint Committee on Cancer (AJCC) stage (p=0.004). In multivariate analyses, low CDK2AP1 expression emerged as an independent prognosticator for worse disease-specific survival (DSS; p=0.037) and local recurrence-free survival (LRFS; p=0.042), along with AJCC stage III-IV (p=0.034, DSS; p=0.029, LRFS).
Low CDK2AP1 expression is common and associated with adverse prognosticators, conferring tumour aggressiveness through cycle cycle, cell growth or apoptosis cellular processes.
细胞周期蛋白依赖性激酶 2 相关蛋白（CDK2AP1）表达水平降低与口腔和食管鳞癌的肿瘤进展相关，但在头颈部癌和鼻咽癌（NPC）患者中研究甚少。
制备兔抗人 CDK2AP1 多克隆抗体。在三种细胞系中检测 CDK2AP1 的免疫印迹，并用免疫组化方法回顾性分析 124 例无初始远处转移且接受一致治疗方案的 NPC 患者的活检标本。
与两个 NPC 来源的 HONE-1 和 TW01 细胞系相比，发育不良的口腔角质形成细胞中 CDK2AP1 的表达水平更高。CDK2AP1 低表达（50.8%）与淋巴结状态（p=0.002）和美国癌症联合委员会（AJCC）分期（p=0.004）相关。多因素分析显示，CDK2AP1 低表达是疾病特异性生存（DSS；p=0.037）和局部无复发生存（LRFS；p=0.042）的独立预后因素，与 AJCC 分期 III-IV 期（p=0.034，DSS；p=0.029，LRFS）相关。
CDK2AP1 低表达常见，与不良预后因素相关，通过周期、细胞生长或细胞凋亡等细胞过程促进肿瘤侵袭性。
