• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

马细胞培养制备中的胶原酶。

Collagenase in equine cell culture preparation.

作者信息

Lang G

出版信息

J Clin Microbiol. 1979 Jun;9(6):731-3. doi: 10.1128/jcm.9.6.731-733.1979.

DOI:10.1128/jcm.9.6.731-733.1979
PMID:227924
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC275389/
Abstract

Equine kidney cells disaggregated by treatment with 0.01% collagenase were used in the preparation of primary monolayer cell cultures. The primary cells could be stored for long periods in liquid nitrogen and subsequently subcultivated. These techniques provided a long-term supply of equine kidney cells, free of apparent contamination, from the kidneys of a single fetus.

摘要

用0.01%胶原酶处理分散得到的马肾细胞用于原代单层细胞培养物的制备。原代细胞可长期保存在液氮中，随后进行传代培养。这些技术提供了来自单个胎儿肾脏的、无明显污染的马肾细胞的长期供应。

相似文献

1
Collagenase in equine cell culture preparation.马细胞培养制备中的胶原酶。
J Clin Microbiol. 1979 Jun;9(6):731-3. doi: 10.1128/jcm.9.6.731-733.1979.
2
Comparative effects of trypsin, collagenase and mechanical harvesting on cell membrane lipids studied in monolayer-cultured endothelial cells and a green monkey kidney cell line.在单层培养的内皮细胞和一种绿猴肾细胞系中研究胰蛋白酶、胶原酶和机械收获对细胞膜脂质的比较影响。
Biochim Biophys Acta. 1985 Jul 30;846(1):120-6. doi: 10.1016/0167-4889(85)90117-x.
3
[Use of collalytine for preparation of monolayer primary cell cultures].[使用胶原酶制备单层原代细胞培养物]
Vopr Virusol. 1978 Jan-Feb(1):118-20.
4
Inhibition of collagenase breakdown of equine corneas by tetanus antitoxin, equine serum and acetylcysteine.破伤风抗毒素、马血清和乙酰半胱氨酸对马角膜胶原酶分解的抑制作用。
Vet Ophthalmol. 2003 Mar;6(1):67-72. doi: 10.1046/j.1463-5224.2003.00271.x.
5
Cell culture of the endocrine pancreas of the mouse in chemically defined media.在化学成分明确的培养基中对小鼠内分泌胰腺进行细胞培养。
In Vitro. 1974 May-Jun;9(6):421-33.
6
Development of a method for isolation of islets from human fetal pancreas.一种从人胎儿胰腺中分离胰岛的方法的开发。
Diabetes. 1989 Jul;38(7):917-24. doi: 10.2337/diab.38.7.917.
7
Separation of human epidermal cells from fibroblasts in primary skin culture.在原代皮肤培养中从成纤维细胞分离人表皮细胞。
Arch Dermatol Forsch. 1975;252(1):39-46. doi: 10.1007/BF00582429.
8
Cell surface distribution and intracellular fate of asialoglycoproteins: a morphological and biochemical study of isolated rat hepatocytes and monolayer cultures.去唾液酸糖蛋白的细胞表面分布及细胞内命运：对分离的大鼠肝细胞和单层培养物的形态学与生物化学研究
J Cell Biol. 1982 Mar;92(3):634-47. doi: 10.1083/jcb.92.3.634.
9
A method for rapid preparation of single-cell suspensions from rat hepatocyte primary cultures on collagen substratum and the mechanism of cell dissociation.一种从胶原蛋白基质上的大鼠肝细胞原代培养物中快速制备单细胞悬液的方法及细胞解离机制。
Acta Med Okayama. 1988 Dec;42(6):351-4. doi: 10.18926/AMO/31002.
10
Isolation, propagation, and cryopreservation of equine articular chondrocytes.马关节软骨细胞的分离、增殖及冷冻保存
Am J Vet Res. 1992 Dec;53(12):2364-70.

本文引用的文献

1
Preservation of cells in tissue culture by glycerol and dimethyl sulphoxide.通过甘油和二甲基亚砜在组织培养中保存细胞。
Nature. 1962 Feb 10;193:548-50. doi: 10.1038/193548a0.
2
ISOLATION AND CULTURE OF THE ISLETS OF LANGERHANS OF THE GUINEA PIG.豚鼠胰岛的分离与培养
Gen Comp Endocrinol. 1965 Jun;5:342-53. doi: 10.1016/0016-6480(65)90059-6.
3
Sialic acid as a structural component of some mammalian tissue cell surfaces.唾液酸作为某些哺乳动物组织细胞表面的一种结构成分。
Nature. 1961 Sep 9;191:1108-9. doi: 10.1038/1911108b0.
4
Propagation of duck hepatitis virus in tissue cultures prepared with collagenase.鸭肝炎病毒在经胶原酶处理的组织培养物中的增殖
J Bacteriol. 1960 Oct;80(4):571-2. doi: 10.1128/jb.80.4.571-572.1960.
5
Amino acid metabolism in mammalian cell cultures.哺乳动物细胞培养中的氨基酸代谢
Science. 1959 Aug 21;130(3373):432-7. doi: 10.1126/science.130.3373.432.
6
Mammalian cell cultures for study of influenza virus. I. Preparation of monolayer cultures with collagenase.用于流感病毒研究的哺乳动物细胞培养。I. 用胶原酶制备单层培养物
Proc Soc Exp Biol Med. 1959 May;101(1):19-22. doi: 10.3181/00379727-101-24818.
7
Established kidney cell lines of normal adult bovine and ovine origin.源自正常成年牛和羊的已建立的肾细胞系。
Proc Soc Exp Biol Med. 1958 Jul;98(3):574-6. doi: 10.3181/00379727-98-24111.
8
Cultivation and behavior in vitro of the normal mammary epithelium of the adult mouse.成年小鼠正常乳腺上皮细胞的体外培养与行为
Anat Rec. 1957 Jan;127(1):117-29. doi: 10.1002/ar.1091270111.
9
Monolayer tissue cultures. I. Preparation and standardization of suspensions of trypsin-dispersed monkey kidney cells.单层组织培养。I. 胰蛋白酶分散的猴肾细胞悬液的制备与标准化
Proc Soc Exp Biol Med. 1954 Feb;85(2):202-5. doi: 10.3181/00379727-85-20830.
10
Plaque formation and isolation of pure lines with poliomyelitis viruses.脊髓灰质炎病毒的噬斑形成及纯系分离
J Exp Med. 1954 Feb;99(2):167-82. doi: 10.1084/jem.99.2.167.