马细胞培养制备中的胶原酶。
Collagenase in equine cell culture preparation.
作者信息
Lang G
出版信息
J Clin Microbiol. 1979 Jun;9(6):731-3. doi: 10.1128/jcm.9.6.731-733.1979.
Abstract
Equine kidney cells disaggregated by treatment with 0.01% collagenase were used in the preparation of primary monolayer cell cultures. The primary cells could be stored for long periods in liquid nitrogen and subsequently subcultivated. These techniques provided a long-term supply of equine kidney cells, free of apparent contamination, from the kidneys of a single fetus.
摘要
用0.01%胶原酶处理分散得到的马肾细胞用于原代单层细胞培养物的制备。原代细胞可长期保存在液氮中，随后进行传代培养。这些技术提供了来自单个胎儿肾脏的、无明显污染的马肾细胞的长期供应。
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