Zhu Fagen, Yu Hongli, Wu Hao, Shi Runjun, Tao Wenting, Qiu Yunying
Pharmacology College of Nanjing University of Chinese Medicine, Nanjing 210029, China.
Zhongguo Zhong Yao Za Zhi. 2012 Apr;37(7):1007-11.
To study the correlation of Pinellia ternata agglutinin (PTA) and toxicity of P. ternata raphides and to find out the toxic mechanism of P. ternata.
PTA has obvious effect of pro-inflammation. The model of rats peritonitis was used to study the dose-toxicity and time-toxicity relationship of the effect by detecting the releases of inflammatory mediators PGE2 in the exudates. The model of Draize rabbit eye test was applied to determine the correlation of PTA and toxicity of raphides by pathological examination.
PTA enhanced the content of PGE2 and protein in rats peritoneal cavities concentration dependently. With PTA concentration increased, PTA enhanced the inflammation induced by raphides to rabbit eyes, but PTA alone had no toxicity response.
PTA had obvious effect of pro-inflammation. The toxic mechanism of P. ternata was PTA induced inflammation only when the raphides pierce into the organization.
研究半夏凝集素（PTA）与半夏针晶毒性的相关性，以明确半夏的毒性机制。
PTA具有明显的促炎作用。采用大鼠腹膜炎模型，通过检测渗出液中炎症介质PGE2的释放，研究其作用的剂量-毒性和时间-毒性关系。应用Draize家兔眼试验模型，通过病理检查确定PTA与针晶毒性的相关性。
PTA浓度依赖性地增加大鼠腹腔内PGE2和蛋白质的含量。随着PTA浓度的增加，PTA增强了针晶对兔眼的炎症诱导作用，但单独的PTA无毒性反应。
PTA具有明显的促炎作用。半夏的毒性机制是仅当针晶刺入组织时，PTA诱导炎症反应。
